| Title |
The effect of different collagen modifications for titanium and titanium nitrite surfaces on functions of gingival fibroblasts
|
|---|---|
| Published in |
Clinical Oral Investigations, March 2016
|
| DOI | 10.1007/s00784-016-1784-5 |
| Pubmed ID | |
| Authors |
U. Ritz, T. Nusselt, A. Sewing, T. Ziebart, K. Kaufmann, A. Baranowski, P.M. Rommens, Alexander Hofmann |
| Abstract |
Targeted modifications of the bulk implant surfaces using bioactive agents provide a promising tool for improvement of the long-term bony and soft tissue integration of dental implants. In this study, we assessed the cellular responses of primary human gingival fibroblasts (HGF) to different surface modifications of titanium (Ti) and titanium nitride (TiN) alloys with type I collagen or cyclic-RGDfK-peptide in order to define a modification improving long-term implants in dental medicine. Employing Ti and TiN implants, we compared the performance of simple dip coating and anodic immobilization of type I collagen that provided collagen layers of two different thicknesses. HGF were seeded on the different coated implants, and adhesion, proliferation, and gene expression were analyzed. Although there were no strong differences in initial cell adhesion between the groups at 2 and 4 hours, we found that all surface modifications induced higher proliferation rates as compared to the unmodified controls. Consistently, gene expression levels of cell adhesion markers (focal adhesion kinase (FAK), integrin beta1, and vinculin), cell differentiation markers (FGFR1, TGFb-R1), extracellular protein markers (type I collagen, vimentin), and cytoskeletal protein marker aktinin-1 were consistently higher in all surface modification groups at two different time points of investigation as compared to the unmodified controls. Our results indicate that simple dip coating of Ti and TiN with collagen is sufficient to induce in vitro cellular responses that are comparable to those of more reliable coating methods like anodic adsorption, chemical cross-linking, or RGD coating. TiN alloys do not possess any positive or adverse effects on HGF. Our results demonstrate a simple, yet effective, method for collagen coating on titanium implants to improve the long term integration and stability of dental implants. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 62 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 2% |
| Italy | 1 | 2% |
| Unknown | 60 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 8 | 13% |
| Researcher | 8 | 13% |
| Student > Doctoral Student | 5 | 8% |
| Student > Postgraduate | 5 | 8% |
| Student > Ph. D. Student | 5 | 8% |
| Other | 10 | 16% |
| Unknown | 21 | 34% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 17 | 27% |
| Materials Science | 6 | 10% |
| Agricultural and Biological Sciences | 5 | 8% |
| Biochemistry, Genetics and Molecular Biology | 4 | 6% |
| Engineering | 3 | 5% |
| Other | 4 | 6% |
| Unknown | 23 | 37% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 March 2016.
All research outputs
#20,315,221
of 22,856,968 outputs
Outputs from Clinical Oral Investigations
#1,019
of 1,409 outputs
Outputs of similar age
#253,606
of 300,258 outputs
Outputs of similar age from Clinical Oral Investigations
#38
of 70 outputs
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