| Title |
Quantification of sialic acids in red meat by UPLC-FLD using indoxylsialosides as internal standards
|
|---|---|
| Published in |
Glycoconjugate Journal, March 2016
|
| DOI | 10.1007/s10719-016-9659-1 |
| Pubmed ID | |
| Authors |
Hong L. Yao, Louis P. Conway, Mao M. Wang, Kun Huang, Li Liu, Josef Voglmeir |
| Abstract |
Herein we describe a UPLC-FLD-based method for the quantification of the sialic acid content of red meat, using a synthetic neuraminic acid derivative as an internal standard. X-Gal-α-2,6-N-propionylneuraminic acid was synthesized via a chemoenzymatic pathway and its hydrolytic stability was characterized. Known quantities of this compound were incubated with samples of red meat under sialic acid-releasing conditions. The released sialic acids were derivatized, analyzed by UPLC-FLD, and the Neu5Ac/Neu5Gc content of the meat sample was determined by comparison with the internal standard. A number of red meats were analyzed by this method with the following results (Neu5Ac μg/g tissue, Neu5Gc μg/g tissue ± s.d.): pork (68 ± 3, 15.2 ± 0.7), beef (69 ± 8, 36 ± 5), lamb (46 ± 2, 33 ± 1), rabbit (59 ± 2, 0.4 ± 0.4), and hare (50 ± 4, 1 ± 1). We envisage that this methodology will find application in investigating the health effects of dietary Neu5Gc. Graphical abstract ᅟ. |
X Demographics
The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Poland | 1 | 20% |
| United Kingdom | 1 | 20% |
| Unknown | 3 | 60% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 3 | 60% |
| Members of the public | 2 | 40% |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 30% |
| Student > Ph. D. Student | 3 | 15% |
| Professor | 1 | 5% |
| Student > Master | 1 | 5% |
| Student > Postgraduate | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 8 | 40% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 6 | 30% |
| Biochemistry, Genetics and Molecular Biology | 3 | 15% |
| Agricultural and Biological Sciences | 2 | 10% |
| Psychology | 1 | 5% |
| Unknown | 8 | 40% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 March 2016.
All research outputs
#15,090,466
of 25,371,288 outputs
Outputs from Glycoconjugate Journal
#619
of 929 outputs
Outputs of similar age
#155,914
of 314,366 outputs
Outputs of similar age from Glycoconjugate Journal
#9
of 13 outputs
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