| Title |
Mycoplasma contamination of cell cultures: Incidence, sources, effects, detection, elimination, prevention
|
|---|---|
| Published in |
Methods in Cell Science, July 2002
|
| DOI | 10.1023/a:1022913015916 |
| Pubmed ID | |
| Authors |
Hans G. Drexler, Cord C. Uphoff |
| Abstract |
The contamination of cell cultures by mycoplasmas remains a major problem in cell culture. Mycoplasmas can produce a virtually unlimited variety of effects in the cultures they infect. These organisms are resistant to most antibiotics commonly employed in cell cultures. Here we provide a concise overview of the current knowledge on: (1) the incidence and sources of mycoplasma contamination in cell cultures, the mycoplasma species most commonly detected in cell cultures, and the effects of mycoplasmas on the function and activities of infected cell cultures; (2) the various techniques available for the detection of mycoplasmas with particular emphasis on the most reliable detection methods; (3) the various methods available for the elimination of mycoplasmas highlighting antibiotic treatment; and (4) the recommended procedures and working protocols for the detection, elimination and prevention of mycoplasma contamination. The availability of accurate, sensitive and reliable detection methods and the application of robust and successful elimination methods provide powerful means for overcoming the problem of mycoplasma contamination in cell cultures. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Israel | 1 | 25% |
| United States | 1 | 25% |
| Canada | 1 | 25% |
| Unknown | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Scientists | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 832 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 6 | <1% |
| United States | 4 | <1% |
| Brazil | 2 | <1% |
| United Kingdom | 2 | <1% |
| Australia | 1 | <1% |
| South Africa | 1 | <1% |
| India | 1 | <1% |
| Switzerland | 1 | <1% |
| Cyprus | 1 | <1% |
| Other | 7 | <1% |
| Unknown | 806 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 181 | 22% |
| Student > Ph. D. Student | 130 | 16% |
| Researcher | 116 | 14% |
| Student > Master | 112 | 13% |
| Student > Postgraduate | 42 | 5% |
| Other | 88 | 11% |
| Unknown | 163 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 212 | 25% |
| Biochemistry, Genetics and Molecular Biology | 206 | 25% |
| Medicine and Dentistry | 57 | 7% |
| Immunology and Microbiology | 39 | 5% |
| Chemistry | 28 | 3% |
| Other | 115 | 14% |
| Unknown | 175 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 388. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 June 2023.
All research outputs
#78,955
of 25,373,627 outputs
Outputs from Methods in Cell Science
#1
of 1,026 outputs
Outputs of similar age
#35
of 47,904 outputs
Outputs of similar age from Methods in Cell Science
#1
of 4 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,026 research outputs from this source. They receive a mean Attention Score of 4.7. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 47,904 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 4 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them