Title |
Electrochemical detection of protein glycosylation using lectin and protein–gold affinity interactions
|
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Published in |
Analyst, January 2016
|
DOI | 10.1039/c6an00528d |
Pubmed ID | |
Authors |
Sharda Yadav, Laura G. Carrascosa, Abu A. I. Sina, Muhammad J. A. Shiddiky, Michelle M. Hill, Matt Trau |
Abstract |
We report a new method for the electrochemical detection of glycosylation on proteins, which relies on lectin-protein interaction on a bare gold electrode. The target protein isolated by immunoaffinity is directly adsorbed onto a gold surface and its glycosylation status is retrieved by subsequent addition of specific lectins. The adsorption and subsequent recognition process is monitored electrochemically in the presence of [Fe(CN)6](3-/4-) redox system. By decoupling target protein capture from glycosylation read-out steps, this approach circumvents unwanted antibody-lectin crosstalk while enabling specific glycosylation detection of a glycoprotein in serum-spiked samples in less than 1 h. |
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Australia | 1 | 50% |
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Demographic breakdown
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Members of the public | 1 | 50% |
Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
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Student > Master | 5 | 28% |
Student > Ph. D. Student | 4 | 22% |
Student > Bachelor | 3 | 17% |
Other | 1 | 6% |
Student > Doctoral Student | 1 | 6% |
Other | 1 | 6% |
Unknown | 3 | 17% |
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Materials Science | 1 | 6% |
Other | 1 | 6% |
Unknown | 3 | 17% |