| Title |
Fluorescence in situ hybridization with high-complexity repeat-free oligonucleotide probes generated by massively parallel synthesis
|
|---|---|
| Published in |
Chromosome Research, October 2011
|
| DOI | 10.1007/s10577-011-9245-0 |
| Pubmed ID | |
| Authors |
Shelagh Boyle, Matthew J. Rodesch, Heather A. Halvensleben, Jeffrey A. Jeddeloh, Wendy A. Bickmore |
| Abstract |
The ability to visualize specific DNA sequences, on chromosomes and in nuclei, by fluorescence in situ hybridization (FISH) is fundamental to many aspects of genetics, genomics and cell biology. Probe selection is currently limited by the availability of DNA clones or the appropriate pool of DNA sequences for PCR amplification. Here, we show that liquid-phase probe pools from sequence capture technology can be adapted to generate fluorescently labelled pools of oligonucleotides that are very effective as repeat-free FISH probes in mammalian cells. As well as detection of small (15 kb) and larger (100 kb) specific loci in both cultured cells and tissue sections, we show that complex oligonucleotide pools can be used as probes to visualize features of nuclear organization. Using this approach, we dramatically reveal the disposition of exons around the outside of a chromosome territory core and away from the nuclear periphery. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 33% |
| United States | 1 | 33% |
| Australia | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 67% |
| Members of the public | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 212 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 3 | 1% |
| Germany | 2 | <1% |
| United States | 2 | <1% |
| Israel | 1 | <1% |
| Portugal | 1 | <1% |
| Russia | 1 | <1% |
| Estonia | 1 | <1% |
| Unknown | 201 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 56 | 26% |
| Researcher | 47 | 22% |
| Student > Master | 18 | 8% |
| Student > Bachelor | 16 | 8% |
| Professor | 15 | 7% |
| Other | 34 | 16% |
| Unknown | 26 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 90 | 42% |
| Biochemistry, Genetics and Molecular Biology | 64 | 30% |
| Computer Science | 7 | 3% |
| Engineering | 6 | 3% |
| Medicine and Dentistry | 4 | 2% |
| Other | 15 | 7% |
| Unknown | 26 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 March 2019.
All research outputs
#3,990,828
of 22,663,150 outputs
Outputs from Chromosome Research
#57
of 507 outputs
Outputs of similar age
#23,033
of 139,136 outputs
Outputs of similar age from Chromosome Research
#2
of 5 outputs
Altmetric has tracked 22,663,150 research outputs across all sources so far. Compared to these this one has done well and is in the 82nd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 507 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.5. This one has done well, scoring higher than 88% of its peers.
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We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.