Lamins are intermediate filament proteins forming a fibrous meshwork, called nuclear lamina, between the inner nuclear membrane and peripheral heterochromatin of metazoan cells. The assembly and incorporation of lamin A/C into the lamina as well as their various functions are still not well understood. Here, we employed designed ankyrin repeat proteins (DARPins) as novel experimental tools for lamin research. We screened for DARPins that specifically bind lamin A/C, interfere with lamin assembly in vitro, and with incorporation of lamin A/C into the native lamina in living cells. Selected DARPins inhibit lamin assembly and delocalize A-type lamins to the nucleoplasm without modifying lamin expression levels or amino acid sequence. Using these lamin binders we demonstrate the importance of proper integration of lamin A/C into the lamina for nuclear mechanical properties and nuclear envelope integrity. Finally, our study provides evidence for cell-type specific differences in lamin functions.
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 March 2016.
All research outputs
#16,721,717
of 25,373,627 outputs
Outputs from Journal of Cell Science
#6,964
of 9,019 outputs
Outputs of similar age
#209,894
of 359,528 outputs
Outputs of similar age from Journal of Cell Science
#127
of 236 outputs
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So far Altmetric has tracked 9,019 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one is in the 19th percentile – i.e., 19% of its peers scored the same or lower than it.
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