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A new virus discovered by immunocapture of double‐stranded RNA, a rapid method for virus enrichment in metagenomic studies

Overview of attention for article published in Molecular Ecology Resources, April 2016
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (51st percentile)

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4 X users

Citations

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37 Dimensions

Readers on

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94 Mendeley
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2 CiteULike
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Title
A new virus discovered by immunocapture of double‐stranded RNA, a rapid method for virus enrichment in metagenomic studies
Published in
Molecular Ecology Resources, April 2016
DOI 10.1111/1755-0998.12525
Pubmed ID
Authors

Arnaud G Blouin, Howard A Ross, Jody Hobson-Peters, Caitlin A O'Brien, Ben Warren, Robin MacDiarmid

Abstract

Next Generation Sequencing technologies enable the rapid identification of viral infection of diseased organisms. However, despite a consistent decrease in sequencing costs, it is difficult to justify their use in large-scale surveys without a virus sequence enrichment technique. Since the majority of plant viruses have an RNA genome, a common approach is to extract the double-stranded RNA (dsRNA) replicative form, to enrich the replicating virus genetic material over the host background. The traditional dsRNA extraction is time-consuming and labour-intensive. We present an alternative method to enrich dsRNA from plant extracts using anti-dsRNA monoclonal antibodies in a pull-down assay. The extracted dsRNA can be amplified by reverse transcriptase polymerase chain reaction and sequenced by Next Generation Sequencing. In our study we have selected three distinct plant hosts: Māori potato (Solanum tuberosum), rengarenga (Arthropodium cirratum) and broad-leaved dock (Rumex obtusifolius) representing a cultivated crop, a New Zealand native ornamental plant and a weed, respectively. Of the sequence data obtained, 31% to 74% of the reads were of viral origin and we identified five viruses including Potato virus Y and Potato virus S in potato; Turnip mosaic virus in rengarenga (a new host record); and in the dock sample Cherry leaf roll virus and a novel virus belonging to the genus Macluravirus. We believe that this new assay represents a significant opportunity to upscale virus ecology studies from environmental, primary industry and / or medical samples. This article is protected by copyright. All rights reserved.

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X Demographics

The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 94 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 1%
Poland 1 1%
Brazil 1 1%
Unknown 91 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 21 22%
Researcher 18 19%
Student > Master 13 14%
Student > Doctoral Student 7 7%
Student > Bachelor 7 7%
Other 18 19%
Unknown 10 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 47 50%
Biochemistry, Genetics and Molecular Biology 19 20%
Medicine and Dentistry 3 3%
Immunology and Microbiology 3 3%
Unspecified 2 2%
Other 7 7%
Unknown 13 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 November 2016.
All research outputs
#14,606,601
of 25,388,353 outputs
Outputs from Molecular Ecology Resources
#1,292
of 1,873 outputs
Outputs of similar age
#148,552
of 311,773 outputs
Outputs of similar age from Molecular Ecology Resources
#22
of 32 outputs
Altmetric has tracked 25,388,353 research outputs across all sources so far. This one is in the 41st percentile – i.e., 41% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,873 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 11.1. This one is in the 29th percentile – i.e., 29% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 311,773 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.
We're also able to compare this research output to 32 others from the same source and published within six weeks on either side of this one. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.