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Genome-wide RNAi Screen Identifies Cohesin Genes as Modifiers of Renewal and Differentiation in Human HSCs

Overview of attention for article published in Cell Reports, March 2016
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  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (97th percentile)
  • High Attention Score compared to outputs of the same age and source (91st percentile)

Mentioned by

news
10 news outlets
blogs
1 blog
twitter
17 X users
facebook
1 Facebook page

Citations

dimensions_citation
72 Dimensions

Readers on

mendeley
129 Mendeley
citeulike
1 CiteULike
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Title
Genome-wide RNAi Screen Identifies Cohesin Genes as Modifiers of Renewal and Differentiation in Human HSCs
Published in
Cell Reports, March 2016
DOI 10.1016/j.celrep.2016.02.082
Pubmed ID
Authors

Roman Galeev, Aurélie Baudet, Praveen Kumar, Alexandra Rundberg Nilsson, Björn Nilsson, Shamit Soneji, Therese Törngren, Åke Borg, Anders Kvist, Jonas Larsson

Abstract

To gain insights into the regulatory mechanisms of hematopoietic stem cells (HSCs), we employed a genome-wide RNAi screen in human cord-blood derived cells and identified candidate genes whose knockdown maintained the HSC phenotype during culture. A striking finding was the identification of members of the cohesin complex (STAG2, RAD21, STAG1, and SMC3) among the top 20 genes from the screen. Upon individual validation of these cohesin genes, we found that their knockdown led to an immediate expansion of cells with an HSC phenotype in vitro. A similar expansion was observed in vivo following transplantation to immunodeficient mice. Transcriptome analysis of cohesin-deficient CD34(+) cells showed an upregulation of HSC-specific genes, demonstrating an immediate shift toward a more stem-cell-like gene expression signature upon cohesin deficiency. Our findings implicate cohesin as a major regulator of HSCs and illustrate the power of global RNAi screens to identify modifiers of cell fate.

X Demographics

X Demographics

The data shown below were collected from the profiles of 17 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 129 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 3 2%
Sweden 1 <1%
Unknown 125 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 34 26%
Researcher 28 22%
Student > Master 14 11%
Student > Bachelor 10 8%
Student > Postgraduate 6 5%
Other 11 9%
Unknown 26 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 43 33%
Agricultural and Biological Sciences 40 31%
Medicine and Dentistry 13 10%
Neuroscience 2 2%
Pharmacology, Toxicology and Pharmaceutical Science 1 <1%
Other 4 3%
Unknown 26 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 85. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 March 2016.
All research outputs
#498,275
of 25,373,627 outputs
Outputs from Cell Reports
#974
of 12,955 outputs
Outputs of similar age
#9,354
of 329,941 outputs
Outputs of similar age from Cell Reports
#22
of 268 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 98th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 12,955 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 30.3. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 329,941 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 268 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 91% of its contemporaries.