| Title |
Decentral gene expression analysis for ER+/Her2− breast cancer: results of a proficiency testing program for the EndoPredict assay
|
|---|---|
| Published in |
Virchows Archiv, February 2012
|
| DOI | 10.1007/s00428-012-1204-4 |
| Pubmed ID | |
| Authors |
Carsten Denkert, Ralf Kronenwett, Werner Schlake, Kerstin Bohmann, Roland Penzel, Karsten E. Weber, Heinz Höfler, Ulrich Lehmann, Peter Schirmacher, Katja Specht, Margaretha Rudas, Hans-Heinrich Kreipe, Peter Schraml, Gudrun Schlake, Zsuzsanna Bago-Horvath, Frank Tiecke, Zsuzsanna Varga, Holger Moch, Marcus Schmidt, Judith Prinzler, Dontscho Kerjaschki, Bruno Valentin Sinn, Berit Maria Müller, Martin Filipits, Christoph Petry, Manfred Dietel |
| Abstract |
Gene expression profiles provide important information about the biology of breast tumors and can be used to develop prognostic tests. However, the implementation of quantitative RNA-based testing in routine molecular pathology has not been accomplished, so far. The EndoPredict assay has recently been described as a quantitative RT-PCR-based multigene expression test to identify a subgroup of hormone-receptor-positive tumors that have an excellent prognosis with endocrine therapy only. To transfer this test from bench to bedside, it is essential to evaluate the test-performance in a multicenter setting in different molecular pathology laboratories. In this study, we have evaluated the EndoPredict (EP) assay in seven different molecular pathology laboratories in Germany, Austria, and Switzerland. A set of ten formalin-fixed paraffin-embedded tumors was tested in the different labs, and the variance and accuracy of the EndoPredict assays were determined using predefined reference values. Extraction of a sufficient amount of RNA and generation of a valid EP score was possible for all 70 study samples (100%). The EP scores measured by the individual participants showed an excellent correlation with the reference values, respectively, as reflected by Pearson correlation coefficients ranging from 0.987 to 0.999. The Pearson correlation coefficient of all values compared to the reference value was 0.994. All laboratories determined EP scores for all samples differing not more than 1.0 score units from the pre-defined references. All samples were assigned to the correct EP risk group, resulting in a sensitivity and specificity of 100%, a concordance of 100%, and a kappa of 1.0. Taken together, the EndoPredict test could be successfully implemented in all seven participating laboratories and is feasible for reliable decentralized assessment of gene expression in luminal breast cancer. |
Mendeley readers
The data shown below were compiled from readership statistics for 70 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 70 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 18 | 26% |
| Student > Master | 12 | 17% |
| Student > Ph. D. Student | 9 | 13% |
| Other | 7 | 10% |
| Student > Bachelor | 5 | 7% |
| Other | 11 | 16% |
| Unknown | 8 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 24 | 34% |
| Agricultural and Biological Sciences | 17 | 24% |
| Biochemistry, Genetics and Molecular Biology | 6 | 9% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 6% |
| Engineering | 2 | 3% |
| Other | 7 | 10% |
| Unknown | 10 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 November 2015.
All research outputs
#4,667,001
of 22,663,150 outputs
Outputs from Virchows Archiv
#176
of 1,934 outputs
Outputs of similar age
#30,581
of 155,494 outputs
Outputs of similar age from Virchows Archiv
#1
of 14 outputs
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