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The ESCRT Complexes

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Cover of 'The ESCRT Complexes'

Table of Contents

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    Book Overview
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    Chapter 1 Analysis of the Archaeal ESCRT Apparatus
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    Chapter 2 Genetic and Cell Biology Methods to Study ESCRTs in Drosophila melanogaster
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    Chapter 3 Functional Analysis of ESCRT-Positive Extracellular Vesicles in the Drosophila Wing Imaginal Disc
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    Chapter 4 Subcellular Localization of ESCRT-II in the Nematode C. elegans by Correlative Light Electron Microscopy
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    Chapter 5 Proximity Ligation Assay (PLA) to Determine the Endosomal Localization of ESCRT Subunit in Virus-Infected Cells
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    Chapter 6 Immuno-localization of ESCRT Proteins in Virus-Infected Cells by Fluorescence and Electron Microscopy
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    Chapter 7 Single Cell Fluorescence Ratio Image Analysis for Studying ESCRT Function in Receptor Trafficking
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    Chapter 8 Genetic and Biochemical Analyses of Yeast ESCRT
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    Chapter 9 Live Imaging of ESCRT Proteins in Microfluidically Isolated Hippocampal Axons
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    Chapter 10 Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High-Resolution Imaging Techniques SIM and Cryo-SXT
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    Chapter 11 Three-Dimensional Surface Rendering of ESCRT Proteins Microscopy Data Using UCSF Chimera Software
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    Chapter 12 Transient Expression of ESCRT Components in Arabidopsis Root Cell Suspension Culture-Derived Protoplasts
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    Chapter 13 Crystallization and Biophysical Approaches for Studying the Interactions Between the Vps4-MIT Domain and ESCRT-III Proteins
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    Chapter 14 Biochemical Approaches to Studying Caenorhabditis elegans ESCRT Functions In Vitro
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    Chapter 15 Purification of Recombinant ESCRT-III Proteins and Their Use in Atomic Force Microscopy and In Vitro Binding and Phosphorylation Assays
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    Chapter 16 Assessment of ESCRT Protein CHMP5 Activity on Client Protein Ubiquitination by Immunoprecipitation and Western Blotting
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    Chapter 17 Purification of Plant ESCRT Proteins for Polyclonal Antibody Production
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    Chapter 18 Genetic and Cytological Methods to Study ESCRT Cell Cycle Function in Fission Yeast
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    Chapter 19 ESCRT Mutant Analysis and Imaging of ESCRT Components in the Model Fungus Ustilago maydis
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    Chapter 20 Genetic Suppressor Screen Using an Inducible FREE1-RNAi Line to Detect ESCRT Genetic Interactors in Arabidopsis thaliana
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    Chapter 21 Screening of Interactions with the ESCRT Machinery by a Gaussia princeps Split Luciferase-Based Complementation Assay
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    Chapter 22 RNA Interference-Mediated Inhibition of ESCRT in Mammalian Cells
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Title
The ESCRT Complexes
Published by
Springer New York, August 2019
DOI 10.1007/978-1-4939-9492-2
ISBNs
978-1-4939-9491-5, 978-1-4939-9492-2
Editors

Culetto, Emmanuel, Legouis, Renaud

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