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Cloning, Expression, and Characterization of a NovelEscherichia coli Thioredoxin*

Overview of attention for article published in Journal of Biological Chemistry, December 1997
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3 patents

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Title
Cloning, Expression, and Characterization of a NovelEscherichia coli Thioredoxin*
Published in
Journal of Biological Chemistry, December 1997
DOI 10.1074/jbc.272.49.30841
Pubmed ID
Authors

Antonio Miranda-Vizuete, Anastasios E. Damdimopoulos, Jan-Åke Gustafsson, Giannis Spyrou

Abstract

Thioredoxin (Trx) is a small ubiquitous protein that displays different functions mainly via redox-mediated processes. We here report the cloning of a gene (trxC) coding for a novel thioredoxin in Escherichia coli as well as the expression and characterization of its product. The gene encodes a protein of 139 amino acids (Trx2) with a calculated molecular mass of 15.5 kDa. Trx2 contains two distinct domains: an N-terminal domain of 32 amino acids including two CXXC motifs and a C-terminal domain, with the conserved active site, Trp-Cys-Gly-Pro-Cys, showing high homology to the prokaryotic thioredoxins. Trx2 together with thioredoxin reductase and NADPH is an efficient electron donor for the essential enzyme ribonucleotide reductase and is also able to reduce the interchain disulfide bridges of insulin. The apparent Km value of Trx2 for thioredoxin reductase is similar to that of the previously characterized E. coli thioredoxin (Trx1). The enzymatic activity of Trx2 as a protein-disulfide reductase is increased by preincubation with dithiothreitol, suggesting that oxidation of cysteine residues other than the ones in the active site might regulate its activity. A truncated form of the protein, lacking the N-terminal domain, is insensitive to the presence of dithiothreitol, further confirming the involvement of the additional cysteine residues in modulating Trx2 activity. In addition, the presence of the N-terminal domain appears to confer heat sensitivity to Trx2, unlike Trx1. Finally, Trx2 is present normally in growing E. coli cells as shown by Western blot analysis.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 56 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
Argentina 1 2%
Unknown 54 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 19 34%
Researcher 9 16%
Student > Bachelor 7 13%
Student > Master 5 9%
Professor > Associate Professor 3 5%
Other 5 9%
Unknown 8 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 24 43%
Biochemistry, Genetics and Molecular Biology 13 23%
Chemistry 4 7%
Immunology and Microbiology 2 4%
Linguistics 1 2%
Other 2 4%
Unknown 10 18%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 January 2018.
All research outputs
#8,534,528
of 25,371,288 outputs
Outputs from Journal of Biological Chemistry
#32,956
of 85,238 outputs
Outputs of similar age
#19,620
of 94,539 outputs
Outputs of similar age from Journal of Biological Chemistry
#300
of 671 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 85,238 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one is in the 15th percentile – i.e., 15% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 94,539 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 17th percentile – i.e., 17% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 671 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.