| Title |
Expression of Tyrosine Hydroxylase is Negatively Regulated Via Prion Protein
|
|---|---|
| Published in |
Neurochemical Research, March 2016
|
| DOI | 10.1007/s11064-016-1885-1 |
| Pubmed ID | |
| Authors |
Marcio Henrique Mello da Luz, Isaias Glezer, Andre Machado Xavier, Marcelo Alberti Paiva da Silva, Jessica Monteiro Volejnik Pino, Thiago Panaro Zamith, Taynara Fernanda Vieira, Bruno Brito Antonio, Hanna Karen Moreira Antunes, Vilma Regina Martins, Kil Sun Lee |
| Abstract |
Cellular prion protein (PrP(C)) is a glycoprotein of the plasma membrane that plays pleiotropic functions by interacting with multiple signaling complexes at the cell surface. Recently, a number of studies have reported the involvement of PrP(C) in dopamine metabolism and signaling, including its interactions with tyrosine hydroxylase (TH) and dopamine receptors. However, the outcomes reported by independent studies are still debatable. Therefore in this study, we investigated the effects of PrP(C) on the TH expression during the differentiation of N2a cells with dibutyryl-cAMP, a well-known cAMP analog that activates TH transcription. Upon differentiation, TH was induced with concomitant reduction of PrP(C) at protein level, but not at mRNA level. shRNA-mediated PrP(C) reduction increased the basal level of TH at both mRNA and protein levels without dibutyryl-cAMP treatment. This phenotype was reversed by re-expression of PrP(C). PrP(C) knockdown also potentiated the effect of dibutyryl-cAMP on TH expression. Our findings suggest that PrP(C) has suppressive effects on TH expression. As a consequence, altered PrP(C) functions may affect the regulation of dopamine metabolism and related neurological disorders. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor > Associate Professor | 3 | 30% |
| Professor | 2 | 20% |
| Student > Doctoral Student | 1 | 10% |
| Student > Bachelor | 1 | 10% |
| Researcher | 1 | 10% |
| Other | 1 | 10% |
| Unknown | 1 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 30% |
| Agricultural and Biological Sciences | 3 | 30% |
| Nursing and Health Professions | 1 | 10% |
| Immunology and Microbiology | 1 | 10% |
| Unknown | 2 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 April 2016.
All research outputs
#4,185,991
of 22,858,915 outputs
Outputs from Neurochemical Research
#274
of 2,098 outputs
Outputs of similar age
#65,353
of 299,392 outputs
Outputs of similar age from Neurochemical Research
#9
of 46 outputs
Altmetric has tracked 22,858,915 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,098 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done well, scoring higher than 82% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 299,392 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 76% of its contemporaries.
We're also able to compare this research output to 46 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.