Title |
Capillary ultrastructure and mitochondrial volume density in skeletal muscle in relation to reduced exercise capacity of patients with intermittent claudication
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Published in |
American Journal of Physiology: Regulatory, Integrative & Comparative Physiology, March 2016
|
DOI | 10.1152/ajpregu.00480.2015 |
Pubmed ID | |
Authors |
Oliver Baum, Eleonora Torchetti, Corinna Malik, Birgitte Hoier, Meegan Walker, Philip J Walker, Adolfo Odriozola, Franziska Graber, Stefan A Tschanz, Jens Bangsbo, Hans Hoppeler, Christopher D Askew, Ylva Hellsten |
Abstract |
Intermittent claudication (IC) is the most commonly reported symptom of peripheral arterial disease (PAD). Impaired limb blood flow is a major casual factor of lower exercise tolerance in PAD, but cannot entirely explain it. We hypothesized that IC is associated with structural changes of the capillary-mitochondria interface that could contribute to the reduction of exercise tolerance in IC-patients. Capillary and mitochondrial morphometry were performed after light and transmission electron microscopy using vastus lateralis muscle biopsies of 14 IC-patients and 10 age-matched controls and peak power output (PPO) was determined for all participants using an incremental single-leg knee-extension protocol. Capillary density was lower (411±90 mm(-2)versus 506±95 mm(-2); P≤0.05) in the biopsies of the IC patients than in those of the controls. The basement membrane (BM) around capillaries was thicker (543±82 nm versus 423±97 nm; P≤0.01) and the volume density of mitochondria was lower (3.51±0.56% versus 4.60±0.74; P≤0.01) in the IC-patients than the controls. In the IC-patients, a higher proportion of capillaries appeared with collapsed slit-like lumen and/or swollen endothelium. PPO was lower (18.5±9.9 W versus 33.5±9.4 W; P≤0.01) in the IC-patients than the controls. We suggest that several structural alterations in skeletal muscle, either collectively or separately, contribute to the reduction of exercise tolerance in IC-patients. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Australia | 2 | 50% |
United Kingdom | 1 | 25% |
Spain | 1 | 25% |
Demographic breakdown
Type | Count | As % |
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Scientists | 3 | 75% |
Members of the public | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 47 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 6 | 13% |
Student > Master | 5 | 11% |
Professor | 5 | 11% |
Student > Ph. D. Student | 4 | 9% |
Student > Bachelor | 4 | 9% |
Other | 12 | 26% |
Unknown | 11 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Sports and Recreations | 9 | 19% |
Biochemistry, Genetics and Molecular Biology | 7 | 15% |
Medicine and Dentistry | 6 | 13% |
Agricultural and Biological Sciences | 5 | 11% |
Nursing and Health Professions | 2 | 4% |
Other | 4 | 9% |
Unknown | 14 | 30% |