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Interleukin-3 greatly expands non-adherent endothelial forming cells with pro-angiogenic properties

Overview of attention for article published in Stem Cell Research, April 2015
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  • Average Attention Score compared to outputs of the same age and source

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1 X user
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1 patent

Citations

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17 Dimensions

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Title
Interleukin-3 greatly expands non-adherent endothelial forming cells with pro-angiogenic properties
Published in
Stem Cell Research, April 2015
DOI 10.1016/j.scr.2015.04.002
Pubmed ID
Authors

Lachlan M. Moldenhauer, Michaelia P. Cockshell, Lachlan Frost, Kate A. Parham, Denis Tvorogov, Lih Y. Tan, Lisa M. Ebert, Katie Tooley, Stephen Worthley, Angel F. Lopez, Claudine S. Bonder

Abstract

Circulating endothelial progenitor cells (EPCs) provide revascularisation for cardiovascular disease and the expansion of these cells opens up the possibility of their use as a cell therapy. Herein we show that interleukin-3 (IL3) strongly expands a population of human non-adherent endothelial forming cells (EXnaEFCs) with low immunogenicity as well as pro-angiogenic capabilities in vivo, making their therapeutic utilisation a realistic option. Non-adherent CD133(+) EFCs isolated from human umbilical cord blood and cultured under different conditions were maximally expanded by day 12 in the presence of IL3 at which time a 350-fold increase in cell number was obtained. Cell surface marker phenotyping confirmed expression of the hematopoietic progenitor cell markers CD133, CD117 and CD34, vascular cell markers VEGFR2 and CD31, dim expression of CD45 and absence of myeloid markers CD14 and CD11b. Functional experiments revealed that EXnaEFCs exhibited classical properties of endothelial cells (ECs), namely binding of Ulex europaeus lectin, up-take of acetylated-low density lipoprotein and contribution to EC tube formation in vitro. These EXnaEFCs demonstrated a pro-angiogenic phenotype within two independent in vivo rodent models. Firstly, a Matrigel plug assay showed increased vascularisation in mice. Secondly, a rat model of acute myocardial infarction demonstrated reduced heart damage as determined by lower levels of serum creatinine and a modest increase in heart functionality. Taken together, these studies show IL3 as a potent growth factor for human CD133(+) cell expansion with clear pro-angiogenic properties (in vitro and in vivo) and thus may provide clinical utility for humans in the future.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Canada 1 4%
Unknown 26 96%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 19%
Student > Ph. D. Student 4 15%
Other 3 11%
Student > Doctoral Student 3 11%
Student > Bachelor 2 7%
Other 4 15%
Unknown 6 22%
Readers by discipline Count As %
Medicine and Dentistry 7 26%
Agricultural and Biological Sciences 5 19%
Biochemistry, Genetics and Molecular Biology 2 7%
Pharmacology, Toxicology and Pharmaceutical Science 2 7%
Chemistry 2 7%
Other 4 15%
Unknown 5 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 September 2017.
All research outputs
#8,261,140
of 25,371,288 outputs
Outputs from Stem Cell Research
#526
of 1,967 outputs
Outputs of similar age
#93,360
of 279,973 outputs
Outputs of similar age from Stem Cell Research
#12
of 19 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one has received more attention than most of these and is in the 66th percentile.
So far Altmetric has tracked 1,967 research outputs from this source. They receive a mean Attention Score of 4.1. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 279,973 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.
We're also able to compare this research output to 19 others from the same source and published within six weeks on either side of this one. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.