Title |
CRISPR/Cas9: an advanced tool for editing plant genomes
|
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Published in |
Transgenic Research, March 2016
|
DOI | 10.1007/s11248-016-9953-5 |
Pubmed ID | |
Authors |
Milan Kumar Samanta, Avishek Dey, Srimonta Gayen |
Abstract |
To meet current challenges in agriculture, genome editing using sequence-specific nucleases (SSNs) is a powerful tool for basic and applied plant biology research. Here, we describe the principle and application of available genome editing tools, including zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeat associated CRISPR/Cas9 system. Among these SSNs, CRISPR/Cas9 is the most recently characterized and rapidly developing genome editing technology, and has been successfully utilized in a wide variety of organisms. This review specifically illustrates the power of CRISPR/Cas9 as a tool for plant genome engineering, and describes the strengths and weaknesses of the CRISPR/Cas9 technology compared to two well-established genome editing tools, ZFNs and TALENs. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 25% |
Canada | 1 | 25% |
United Kingdom | 1 | 25% |
Unknown | 1 | 25% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 75% |
Scientists | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Portugal | 1 | <1% |
Germany | 1 | <1% |
Indonesia | 1 | <1% |
Chile | 1 | <1% |
United Kingdom | 1 | <1% |
Sri Lanka | 1 | <1% |
Argentina | 1 | <1% |
United States | 1 | <1% |
Unknown | 240 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 53 | 21% |
Student > Ph. D. Student | 34 | 14% |
Student > Master | 32 | 13% |
Student > Bachelor | 31 | 13% |
Student > Doctoral Student | 11 | 4% |
Other | 31 | 13% |
Unknown | 56 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 102 | 41% |
Biochemistry, Genetics and Molecular Biology | 59 | 24% |
Immunology and Microbiology | 5 | 2% |
Medicine and Dentistry | 4 | 2% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 1% |
Other | 10 | 4% |
Unknown | 65 | 26% |