| Title |
Methyl labeling and TROSY NMR spectroscopy of proteins expressed in the eukaryote Pichia pastoris
|
|---|---|
| Published in |
Journal of Biomolecular NMR, May 2015
|
| DOI | 10.1007/s10858-015-9939-2 |
| Pubmed ID | |
| Authors |
Lindsay Clark, Jacob A. Zahm, Rustam Ali, Maciej Kukula, Liangqiao Bian, Steven M. Patrie, Kevin H. Gardner, Michael K. Rosen, Daniel M. Rosenbaum |
| Abstract |
(13)C Methyl TROSY NMR spectroscopy has emerged as a powerful method for studying the dynamics of large systems such as macromolecular assemblies and membrane proteins. Specific (13)C labeling of aliphatic methyl groups and perdeuteration has been limited primarily to proteins expressed in E. coli, preventing studies of many eukaryotic proteins of physiological and biomedical significance. We demonstrate the feasibility of efficient (13)C isoleucine δ1-methyl labeling in a deuterated background in an established eukaryotic expression host, Pichia pastoris, and show that this method can be used to label the eukaryotic protein actin, which cannot be expressed in bacteria. This approach will enable NMR studies of previously intractable targets. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 71 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 1% |
| Unknown | 70 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 22 | 31% |
| Researcher | 15 | 21% |
| Student > Master | 10 | 14% |
| Professor | 6 | 8% |
| Student > Bachelor | 2 | 3% |
| Other | 7 | 10% |
| Unknown | 9 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 29 | 41% |
| Agricultural and Biological Sciences | 16 | 23% |
| Chemistry | 13 | 18% |
| Unspecified | 1 | 1% |
| Physics and Astronomy | 1 | 1% |
| Other | 1 | 1% |
| Unknown | 10 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 April 2016.
All research outputs
#15,692,595
of 23,318,744 outputs
Outputs from Journal of Biomolecular NMR
#386
of 618 outputs
Outputs of similar age
#158,455
of 268,215 outputs
Outputs of similar age from Journal of Biomolecular NMR
#2
of 13 outputs
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So far Altmetric has tracked 618 research outputs from this source. They receive a mean Attention Score of 2.9. This one is in the 27th percentile – i.e., 27% of its peers scored the same or lower than it.
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