| Title |
Cryopreservation of rat islets of Langerhans by vitrification
|
|---|---|
| Published in |
Journal of Artificial Organs, March 2012
|
| DOI | 10.1007/s10047-012-0635-7 |
| Pubmed ID | |
| Authors |
Hiromi Sasamoto, Midori Futami, Yusuke Ando, Shuhei Nakaji |
| Abstract |
Cryopreservation could be a possible means of addressing the shortage of islets of Langerhans. We investigated the effects of EDT324 solution on the vitrification of isolated rat islets of Langerhans. Rat pancreatic islets were cryopreserved in 10% DMSO by a slow-rate freezing method or were cryopreserved in EDT324 solution by vitrification. The cryopreserved islets were compared in terms of viability, stimulation index and metabolic function after transplantation. After cryopreservation, the viability and stimulation of islets stored in EDT324 were 92.4% and 6.4, respectively, and were higher than islets stored by slow freezing (72.5% and 1.5, respectively). Streptozotocin-induced diabetic rats were transplanted with islets cryopreserved in EDT324, which corrected diabetes and achieved euglycemia within 2 days after transplantation. These results indicate that EDT324 allows successful cryopreservation of rat islets for long-term storage as an alternative solution to traditionally used solutions, such as 10% DMSO. Transplantation of cryopreserved islets into diabetic rats can achieve euglycemia. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 6% |
| Unknown | 16 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 24% |
| Lecturer | 3 | 18% |
| Student > Doctoral Student | 3 | 18% |
| Student > Bachelor | 2 | 12% |
| Student > Ph. D. Student | 2 | 12% |
| Other | 2 | 12% |
| Unknown | 1 | 6% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 41% |
| Engineering | 4 | 24% |
| Biochemistry, Genetics and Molecular Biology | 1 | 6% |
| Veterinary Science and Veterinary Medicine | 1 | 6% |
| Economics, Econometrics and Finance | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 2 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 March 2012.
All research outputs
#18,305,470
of 22,663,969 outputs
Outputs from Journal of Artificial Organs
#161
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#121,103
of 156,014 outputs
Outputs of similar age from Journal of Artificial Organs
#4
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