| Title |
Quantification of urinary AICAR concentrations as a matter of doping controls
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, March 2010
|
| DOI | 10.1007/s00216-010-3560-8 |
| Pubmed ID | |
| Authors |
Andreas Thomas, Simon Beuck, Jens Christian Eickhoff, Sven Guddat, Oliver Krug, Matthias Kamber, Wilhelm Schänzer, Mario Thevis |
| Abstract |
Influencing the endurance in elite sports is one of the key points in modern sports science. Recently, a new class of prohibited substances reached in the focus of doping control laboratories and their misuse was classified as gene doping. The adenosine monophosphate activated protein kinase activator 5-amino-4-imidazolecarboxyamide ribonucleoside (AICAR) was found to significantly enhance the endurance even in sedentary mice after treatment. Due to endogenous production of AICAR in healthy humans, considerable amounts were present in the circulation and, thus, were excreted into urine. Considering these facts, the present study was initiated to fix reference values of renally cleared AICAR in elite athletes. Therefore a quantitative analytical method by means of isotope-dilution liquid chromatography (analytical column: C6-phenyl) coupled to tandem mass spectrometry, after a sample preparation consisting of a gentle dilution of native urine, was developed. Doping control samples of 499 athletes were analysed, and AICAR concentrations in urine were determined. The mean AICAR value for all samples was 2,186 ng/mL with a standard deviation of 1,655 ng/mL. Concentrations were found to differ depending on gender, type of sport and type of sample collection (in competition/out of competition). The method was fully validated for quantitative purposes considering the parameters linearity, inter- (12%, 7% and 10%) and intraday precision (14%, 9% and 12%) at low, mid and high concentration, robustness, accuracy (approx. 100%), limit of quantification (100 ng/mL), stability and ion suppression effects, employing an in-house synthesised (13)C(5)-labelled AICAR as internal standard. |
X Demographics
The data shown below were collected from the profiles of 10 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 3 | 30% |
| Spain | 2 | 20% |
| Unknown | 5 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 9 | 90% |
| Scientists | 1 | 10% |
Mendeley readers
The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Poland | 2 | 5% |
| Hungary | 1 | 2% |
| Unknown | 39 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 19% |
| Researcher | 8 | 19% |
| Student > Master | 5 | 12% |
| Lecturer | 3 | 7% |
| Professor | 2 | 5% |
| Other | 3 | 7% |
| Unknown | 13 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 14 | 33% |
| Sports and Recreations | 3 | 7% |
| Biochemistry, Genetics and Molecular Biology | 2 | 5% |
| Arts and Humanities | 2 | 5% |
| Neuroscience | 2 | 5% |
| Other | 5 | 12% |
| Unknown | 14 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 February 2022.
All research outputs
#3,113,255
of 25,576,275 outputs
Outputs from Analytical & Bioanalytical Chemistry
#300
of 9,672 outputs
Outputs of similar age
#11,861
of 103,209 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#8
of 54 outputs
Altmetric has tracked 25,576,275 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 9,672 research outputs from this source. They receive a mean Attention Score of 3.1. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 103,209 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 88% of its contemporaries.
We're also able to compare this research output to 54 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.