| Title |
DNA Methylation Assay for X-Chromosome Inactivation in Female Human iPS Cells
|
|---|---|
| Published in |
Stem Cell Reviews and Reports, March 2011
|
| DOI | 10.1007/s12015-011-9238-6 |
| Pubmed ID | |
| Authors |
Lesli A. Kiedrowski, Gordana Raca, Jennifer J. Laffin, Benjamin S. Nisler, Kimberly Leonhard, Erik McIntire, Karen Dyer Mongomery |
| Abstract |
Remarkable interest in the epigenetic status of human induced pluripotent stem (iPS) cells inspired numerous studies of their X-inactivation patterns. However, both the presence and the absence of X-inactivation have been described to date in undifferentiated iPS cells. The reasons for the discordant results between different studies are unclear, and further X-inactivation testing is warranted for all female human iPS cell lines. Some of the inconsistency in the current data most likely results from the use of different X-inactivation assays by different authors. We provide a detailed protocol for a simple, reliable and affordable X-inactivation assay based on promoter methylation and CAG-repeat polymorphism in the human androgen receptor (AR) gene at Xq11.2. This assay is commonly used in clinical genetic laboratories and we propose that it could be ideal for routine assessment and monitoring of the X-inactivation status in female human iPS cell lines. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 5% |
| Brazil | 1 | 2% |
| Portugal | 1 | 2% |
| Japan | 1 | 2% |
| China | 1 | 2% |
| Unknown | 36 | 86% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 16 | 38% |
| Researcher | 10 | 24% |
| Student > Bachelor | 4 | 10% |
| Student > Postgraduate | 3 | 7% |
| Professor | 2 | 5% |
| Other | 5 | 12% |
| Unknown | 2 | 5% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 20 | 48% |
| Biochemistry, Genetics and Molecular Biology | 8 | 19% |
| Medicine and Dentistry | 5 | 12% |
| Neuroscience | 2 | 5% |
| Immunology and Microbiology | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 5 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 March 2012.
All research outputs
#20,657,128
of 25,374,917 outputs
Outputs from Stem Cell Reviews and Reports
#821
of 1,036 outputs
Outputs of similar age
#106,435
of 120,010 outputs
Outputs of similar age from Stem Cell Reviews and Reports
#18
of 19 outputs
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