| Title |
Production of small RNAs by mammalian Dicer
|
|---|---|
| Published in |
Pflügers Archiv - European Journal of Physiology, April 2016
|
| DOI | 10.1007/s00424-016-1817-6 |
| Pubmed ID | |
| Authors |
Eliska Svobodova, Jana Kubikova, Petr Svoboda |
| Abstract |
MicroRNA (miRNA) and RNA interference (RNAi) pathways employ RNase III Dicer for the biogenesis of small RNAs guiding post-transcriptional repression. Requirements for Dicer activity differ in the two pathways. The biogenesis of miRNAs requires a single Dicer cleavage of a short hairpin precursor to produce a small RNA with a precisely defined sequence, while small RNAs in RNAi come from a processive cleavage of a long double-stranded RNA (dsRNA) into a pool of small RNAs with different sequences. While Dicer is generally conserved among eukaryotes, its substrate recognition, cleavage, and biological roles differ. In Metazoa, a single Dicer can function as a universal factor for RNAi and miRNA pathways or as a factor adapted specifically for one of the pathways. In this review, we focus on the structure, function, and evolution of mammalian Dicer. We discuss key structural features of Dicer and other factors defining Dicer substrate repertoire and biological functions in mammals in comparison with invertebrate models. The key for adaptation of Dicer for miRNA or RNAi pathways is the N-terminal helicase, a dynamically evolving Dicer domain. Its functionality differs between mammals and invertebrates: the mammalian Dicer is well adapted to produce miRNAs while its ability to support RNAi is limited. |
X Demographics
The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 20% |
| France | 1 | 20% |
| Unknown | 3 | 60% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 60% |
| Scientists | 2 | 40% |
Mendeley readers
The data shown below were compiled from readership statistics for 112 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | <1% |
| Norway | 1 | <1% |
| Unknown | 110 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 24 | 21% |
| Student > Master | 16 | 14% |
| Student > Bachelor | 15 | 13% |
| Researcher | 15 | 13% |
| Student > Doctoral Student | 6 | 5% |
| Other | 12 | 11% |
| Unknown | 24 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 51 | 46% |
| Agricultural and Biological Sciences | 25 | 22% |
| Immunology and Microbiology | 3 | 3% |
| Neuroscience | 2 | 2% |
| Psychology | 1 | <1% |
| Other | 3 | 3% |
| Unknown | 27 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 April 2016.
All research outputs
#13,824,255
of 23,815,455 outputs
Outputs from Pflügers Archiv - European Journal of Physiology
#1,208
of 1,973 outputs
Outputs of similar age
#146,129
of 302,945 outputs
Outputs of similar age from Pflügers Archiv - European Journal of Physiology
#6
of 24 outputs
Altmetric has tracked 23,815,455 research outputs across all sources so far. This one is in the 41st percentile – i.e., 41% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,973 research outputs from this source. They receive a mean Attention Score of 5.0. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 302,945 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.
We're also able to compare this research output to 24 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 75% of its contemporaries.