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Genotypic and phenotypic identification of Aeromonas species and CphA-mediated carbapenem resistance in Queensland, Australia

Overview of attention for article published in Diagnostic Microbiology & Infectious Disease, March 2016
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Title
Genotypic and phenotypic identification of Aeromonas species and CphA-mediated carbapenem resistance in Queensland, Australia
Published in
Diagnostic Microbiology & Infectious Disease, March 2016
DOI 10.1016/j.diagmicrobio.2016.02.005
Pubmed ID
Authors

Holly A. Sinclair, Claire Heney, Hanna E. Sidjabat, Narelle M. George, Haakon Bergh, Snehal N. Anuj, Graeme R. Nimmo, David L. Paterson

Abstract

Infection caused by Aeromonas spp. ranges from superficial wound infection to life-threatening septicemia. Carbapenem resistance due to metallo-beta-lactamase, CphA encoded by the cphA gene, is a significant problem. This study defines Aeromonas spp. causing clinical disease in Queensland, Australia. Phenotypic tests for carbapenemase detection were assessed. One hundred Aeromonas isolates from blood (22), wound (46), sterile sites (11), stool (18), eye (2), and sputum (1) were characterized by rpoB and gyrB sequencing. Meropenem susceptibility by VITEK2, disk diffusion, and E-test MIC were determined. Carbapenemase production was assessed by Carba NP test and cphA by PCR. Gene sequencing identified isolates as Aeromonas dhakensis (39), Aeromonas veronii (21), Aeromonas hydrophila (20), Aeromonas caviae (14), Aeromonas jandaei (4), Aeromonas bestiarum (1), and Aeromonas sanarellii (1). Disk diffusion and E-test failed to detect resistance in isolates with presence of cphA. Carba NP was performed with 97.4% sensitivity and 95.7% specificity. Carbapenem resistance gene cphA was detected in A. veronii (21; 100%), A. hydrophila (18; 90%), A. dhakensis (34; 87.2%), A. jandaei (3; 75%), and A. bestiarum (1; 100%) but not A. caviae. We found that A. dhakensis was the predominant species, a previously unrecognized pathogen in this region.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 69 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 69 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 18 26%
Student > Bachelor 10 14%
Professor 7 10%
Researcher 6 9%
Student > Ph. D. Student 6 9%
Other 13 19%
Unknown 9 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 13 19%
Agricultural and Biological Sciences 13 19%
Immunology and Microbiology 12 17%
Medicine and Dentistry 8 12%
Veterinary Science and Veterinary Medicine 4 6%
Other 6 9%
Unknown 13 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 June 2022.
All research outputs
#17,286,379
of 25,374,647 outputs
Outputs from Diagnostic Microbiology & Infectious Disease
#1,475
of 2,303 outputs
Outputs of similar age
#191,326
of 314,783 outputs
Outputs of similar age from Diagnostic Microbiology & Infectious Disease
#8
of 30 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,303 research outputs from this source. They receive a mean Attention Score of 3.9. This one is in the 27th percentile – i.e., 27% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 314,783 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 30 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.