| Title |
The de-ubiquitinase UCHL1 promotes gastric cancer metastasis via the Akt and Erk1/2 pathways
|
|---|---|
| Published in |
Tumor Biology, May 2015
|
| DOI | 10.1007/s13277-015-3566-0 |
| Pubmed ID | |
| Authors |
Yu-yu Gu, Mei Yang, Mei Zhao, Qing Luo, Lin Yang, Hua Peng, Jia Wang, Sheng-kai Huang, Zhao-xu Zheng, Xing-hua Yuan, Ping Liu, Chang-zhi Huang |
| Abstract |
Ubiquitin C-terminal hydrolase-L1 (UCHL1) is a de-ubiquitinating enzyme, which enzymatic activity relies on the C90 site. The function of UCHL1 is controversial in different types of cancer, and its role in gastric cancer progression remains unclear. In this study, immunohistochemistry staining was applied to detect the expression of UCHL1 in primary gastric cancer and liver metastases from gastric cancer. MKN45 and BGC823 cell lines with stable expression of de-ubiquitinase active UCHL1 or inactive UCHL1-variant C90S were established by lentiviral infection. The effect of UCHL1 on cell proliferation was evaluated by MTT and colony formation assays. The abilities of cell migration and invasion were determined by transwell assay. Protein expression levels were determined by Western blot. The results indicated that UCHL1 had a significantly higher positive expression rate in liver metastases from gastric cancer compared with primary gastric cancer. Overexpression of UCHL1 in MKN45 and BGC823 cells promoted cell proliferation, migration, and invasion depending on its de-ubiquitinase activity. UCHL1 activated Akt and Erk1/2, which process also required enzymatic activity and was necessary for mediating cell migration and invasion. These findings demonstrated that UCHL1 promoted cell proliferation, migration, and invasion depending on its de-ubiquitinase activity by activating Akt and Erk1/2, which may account for its higher positive expression rate in liver metastases from gastric cancer. UCHL1 could be a candidate biomarker and a therapeutic target for gastric cancer metastasis. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 2 | 7% |
| France | 1 | 4% |
| Sweden | 1 | 4% |
| Unknown | 24 | 86% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 32% |
| Student > Master | 7 | 25% |
| Researcher | 5 | 18% |
| Student > Bachelor | 1 | 4% |
| Professor > Associate Professor | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 4 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 25% |
| Biochemistry, Genetics and Molecular Biology | 5 | 18% |
| Medicine and Dentistry | 4 | 14% |
| Chemistry | 2 | 7% |
| Immunology and Microbiology | 2 | 7% |
| Other | 3 | 11% |
| Unknown | 5 | 18% |
Attention Score in Context
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All research outputs
#20,318,358
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Outputs from Tumor Biology
#1,834
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#222,883
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Outputs of similar age from Tumor Biology
#104
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