| Title |
Quantitative imaging of mitochondrial and cytosolic free zinc levels in an in vitro model of ischemia/reperfusion
|
|---|---|
| Published in |
Journal of Bioenergetics and Biomembranes, March 2012
|
| DOI | 10.1007/s10863-012-9427-2 |
| Pubmed ID | |
| Authors |
Bryan J. McCranor, Rebecca A. Bozym, Michele I. Vitolo, Carol A. Fierke, Linda Bambrick, Brian M. Polster, Gary Fiskum, Richard B. Thompson |
| Abstract |
The role of zinc ion in cytotoxicity following ischemic stroke, prolonged status epilepticus, and traumatic brain injury remains controversial, but likely is the result of mitochondrial dysfunction. We describe an excitation ratiometric fluorescence biosensor based on human carbonic anhydrase II variants expressed in the mitochondrial matrix, permitting free zinc levels to be quantitatively imaged therein. We observed an average mitochondrial matrix free zinc concentration of 0.2 pM in the PC12 rat pheochromacytoma cell culture line. Cytoplasmic and mitochondrial free zinc levels were imaged in a cellular oxygen glucose deprivation (OGD) model of ischemia/reperfusion. We observed a significant increase in mitochondrial zinc 1 h following 3 h OGD, at a time point when cytosolic zinc levels were depressed. Following the increase, mitochondrial zinc levels returned to physiological levels, while cytosolic zinc increased gradually over a 24 h time period in viable cells. The increase in intramitochondrial zinc observed during reoxygenation after OGD may contribute to bioenergetic dysfunction and cell death that occurs with both in vitro and in vivo models of reperfusion. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 58 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 58 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 16% |
| Student > Master | 9 | 16% |
| Researcher | 7 | 12% |
| Professor | 4 | 7% |
| Student > Postgraduate | 4 | 7% |
| Other | 13 | 22% |
| Unknown | 12 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 13 | 22% |
| Chemistry | 9 | 16% |
| Agricultural and Biological Sciences | 8 | 14% |
| Medicine and Dentistry | 4 | 7% |
| Psychology | 2 | 3% |
| Other | 5 | 9% |
| Unknown | 17 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 March 2012.
All research outputs
#16,188,009
of 23,867,274 outputs
Outputs from Journal of Bioenergetics and Biomembranes
#320
of 466 outputs
Outputs of similar age
#104,907
of 162,536 outputs
Outputs of similar age from Journal of Bioenergetics and Biomembranes
#11
of 16 outputs
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