| Title |
The application of multiplex PCR to detect seven different DNA targets in group B streptococci
|
|---|---|
| Published in |
Folia Microbiologica, March 2012
|
| DOI | 10.1007/s12223-012-0108-7 |
| Pubmed ID | |
| Authors |
Tomasz Gosiewski, Monika Brzychczy-Włoch, Piotr B. Heczko |
| Abstract |
Group B Streptococcus (GBS) causes severe infections in infants and in immunocompromised adults. GBS pathogenicity varies between and within serotypes, with considerable variation in genetic content between strains. For this reason, it is important to be able to carry out immediate and comprehensive diagnostics of these infections. Seven genes important for screening of GBS infection were detected: cfb gene encoding the CAMP factor presented in every GBS; the cps operon genes such as cps1aH, cps1a/2/3IJ, and cps5O specific for capsular polysaccharide types Ia, III, and V, respectively; macrolide resistance genes ermB and mefA/E; and the gbs2018 S10 region specific for ST17 hypervirulent clone. Standardization of multiplex PCR with the use of seven primer pairs was performed on 81 bacterial strains representing different GBS isolates (n = 75) and other Gram-positive cocci (n = 10). Multiplex PCR can be used as an effective screening method to detect different sequences important for the screening of GBS infection. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Sweden | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Slovenia | 1 | 2% |
| Unknown | 40 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 10 | 24% |
| Student > Bachelor | 9 | 22% |
| Student > Ph. D. Student | 6 | 15% |
| Professor > Associate Professor | 3 | 7% |
| Student > Doctoral Student | 2 | 5% |
| Other | 5 | 12% |
| Unknown | 6 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 9 | 22% |
| Agricultural and Biological Sciences | 9 | 22% |
| Biochemistry, Genetics and Molecular Biology | 5 | 12% |
| Immunology and Microbiology | 4 | 10% |
| Environmental Science | 2 | 5% |
| Other | 5 | 12% |
| Unknown | 7 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 March 2012.
All research outputs
#15,242,707
of 22,663,969 outputs
Outputs from Folia Microbiologica
#453
of 736 outputs
Outputs of similar age
#100,159
of 156,636 outputs
Outputs of similar age from Folia Microbiologica
#2
of 5 outputs
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So far Altmetric has tracked 736 research outputs from this source. They receive a mean Attention Score of 2.9. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
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