Title |
Single particle analysis of herpes simplex virus: comparing the dimensions of one and the same virions via atomic force and scanning electron microscopy
|
---|---|
Published in |
Analytical & Bioanalytical Chemistry, April 2016
|
DOI | 10.1007/s00216-016-9492-1 |
Pubmed ID | |
Authors |
Evelyn Kämmer, Isabell Götz, Thomas Bocklitz, Stephan Stöckel, Andrea Dellith, Dana Cialla-May, Karina Weber, Roland Zell, Jan Dellith, Volker Deckert, Jürgen Popp |
Abstract |
Currently, two types of direct methods to characterize and identify single virions are available: electron microscopy (EM) and scanning probe techniques, especially atomic force microscopy (AFM). AFM in particular provides morphologic information even of the ultrastructure of viral specimens without the need to cultivate the virus and to invasively alter the sample prior to the measurements. Thus, AFM can play a critical role as a frontline method in diagnostic virology. Interestingly, varying morphological parameters for virions of the same type can be found in the literature, depending on whether AFM or EM was employed and according to the respective experimental conditions during the AFM measurements. Here, an inter-methodological proof of principle is presented, in which the same single virions of herpes simplex virus 1 were probed by AFM previously and after they were measured by scanning electron microscopy (SEM). Sophisticated chemometric analyses then allowed a calculation of morphological parameters of the ensemble of single virions and a comparison thereof. A distinct decrease in the virions' dimensions was found during as well as after the SEM analyses and could be attributed to the sample preparation for the SEM measurements. Graphical abstract The herpes simplex virus is investigated with scanning electron and atomic force microscopy in view of varying dimensions. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 67% |
Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 22 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 8 | 36% |
Researcher | 5 | 23% |
Professor | 2 | 9% |
Other | 2 | 9% |
Student > Doctoral Student | 2 | 9% |
Other | 1 | 5% |
Unknown | 2 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 5 | 23% |
Biochemistry, Genetics and Molecular Biology | 3 | 14% |
Agricultural and Biological Sciences | 2 | 9% |
Computer Science | 2 | 9% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
Other | 7 | 32% |
Unknown | 2 | 9% |