Continuous
monitoring of bacterial growth in aqueous media is a
crucial process in academic research as well as in the biotechnology
industry. Bacterial growth is usually monitored by measuring the optical
density of bacteria in liquid media, using benchtop spectrophotometers.
Due to the large form factor of the existing spectrophotometers, they
cannot be used for live monitoring of the bacteria inside bacterial
incubation chambers. Additionally, the use of benchtop spectrometers
for continuous monitoring requires multiple samplings and is labor
intensive. To overcome these challenges, we have developed an optical
density measuring device (ODX) by modifying a generic fitness tracker.
The resulting ODX device is an ultraportable and low-cost device that
can be used inside bacterial incubators for real-time monitoring even
while shaking is in progress. We evaluated the performance of ODX
with different bacterial types and growth conditions and compared
it with a commercial benchtop spectrophotometer. In all cases, ODX
showed comparable performance to that of the standard benchtop spectrophotometer.
Finally, we demonstrate a simple and useful smartphone application
whereby the user is notified when the bacterial concentration reaches
the targeted value. Due to its potential for automation and mass production,
we believe that the ODX has a wide range of applications in biotechnology
research and industry.