| Title |
l-Glutamine regulates amino acid utilization by intestinal bacteria
|
|---|---|
| Published in |
Amino Acids, March 2012
|
| DOI | 10.1007/s00726-012-1264-4 |
| Pubmed ID | |
| Authors |
Zhao-Lai Dai, Xi-Long Li, Peng-Bin Xi, Jing Zhang, Guoyao Wu, Wei-Yun Zhu |
| Abstract |
Catabolism of amino acids (AA) by intestinal bacteria greatly affects their bioavailability in the systemic circulation and the health of animals and humans. This study tests the novel hypothesis that L-glutamine regulates AA utilization by luminal bacteria of the small intestine. Pure bacterial strains (Streptococcus sp., Escherichia coli and Klebsiella sp.) and mixed bacterial cultures derived from the jejunum or ileum of pigs were cultured in the presence of 0-5 mM L-glutamine under anaerobic conditions. After 3 h of incubation, samples were taken for the determination of AA utilization. Results showed concentration-dependent increases in the utilization of glutamine in parallel with the increased conversion of glutamine into glutamate in all the bacteria. Complete utilization of asparagine, aspartate and serine was observed in pure bacterial strains after the 3-h incubation. The addition of glutamine reduced the net utilization of asparagine by both jejunal and ileal mixed bacteria. Net utilization of lysine, leucine, valine, ornithine and serine by jejunal or ileal mixed bacteria decreased with the addition of glutamine in a concentration-dependent manner. Collectively, glutamine dynamically modulates the bacterial metabolism of the arginine family of AA as well as the serine and aspartate families of AA and reduced the catabolism of most AA (including nutritionally essential and nonessential AA) in jejunal or ileal mixed bacteria. The beneficial effects of glutamine on gut nutrition and health may involve initiation of the signaling pathways related to AA metabolism in the luminal bacteria of the small intestine. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 50% |
| Practitioners (doctors, other healthcare professionals) | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 104 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | <1% |
| Italy | 1 | <1% |
| Unknown | 102 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 22 | 21% |
| Student > Ph. D. Student | 16 | 15% |
| Researcher | 15 | 14% |
| Student > Bachelor | 11 | 11% |
| Student > Doctoral Student | 5 | 5% |
| Other | 12 | 12% |
| Unknown | 23 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 36 | 35% |
| Medicine and Dentistry | 10 | 10% |
| Biochemistry, Genetics and Molecular Biology | 10 | 10% |
| Nursing and Health Professions | 5 | 5% |
| Veterinary Science and Veterinary Medicine | 4 | 4% |
| Other | 12 | 12% |
| Unknown | 27 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 May 2012.
All research outputs
#17,489,487
of 25,654,806 outputs
Outputs from Amino Acids
#1,101
of 1,624 outputs
Outputs of similar age
#113,452
of 173,165 outputs
Outputs of similar age from Amino Acids
#19
of 27 outputs
Altmetric has tracked 25,654,806 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
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We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one is in the 22nd percentile – i.e., 22% of its contemporaries scored the same or lower than it.