| Title |
The Hevea brasiliensis XIP aquaporin subfamily: genomic, structural and functional characterizations with relevance to intensive latex harvesting
|
|---|---|
| Published in |
Plant Molecular Biology, April 2016
|
| DOI | 10.1007/s11103-016-0462-y |
| Pubmed ID | |
| Authors |
David Lopez, Maroua Ben Amira, Daniel Brown, Beatriz Muries, Nicole Brunel-Michac, Sylvain Bourgerie, Benoit Porcheron, Remi Lemoine, Hervé Chrestin, Ewan Mollison, Alessandra Di Cola, Lorenzo Frigerio, Jean-Louis Julien, Aurélie Gousset-Dupont, Boris Fumanal, Philippe Label, Valérie Pujade-Renaud, Daniel Auguin, Jean-Stéphane Venisse |
| Abstract |
X-Intrinsic Proteins (XIP) were recently identified in a narrow range of plants as a full clade within the aquaporins. These channels reportedly facilitate the transport of a wide range of hydrophobic solutes. The functional roles of XIP in planta remain poorly identified. In this study, we found three XIP genes (HbXIP1;1, HbXIP2;1 and HbXIP3;1) in the Hevea brasiliensis genome. Comprehensive bioinformatics, biochemical and structural analyses were used to acquire a better understanding of this AQP subfamily. Phylogenetic analysis revealed that HbXIPs clustered into two major groups, each distributed in a specific lineage of the order Malpighiales. Tissue-specific expression profiles showed that only HbXIP2;1 was expressed in all the vegetative tissues tested (leaves, stem, bark, xylem and latex), suggesting that HbXIP2;1 could take part in a wide range of cellular processes. This is particularly relevant to the rubber-producing laticiferous system, where this isoform was found to be up-regulated during tapping and ethylene treatments. Furthermore, the XIP transcriptional pattern is significantly correlated to latex production level. Structural comparison with SoPIP2;1 from Spinacia oleracea species provides new insights into the possible role of structural checkpoints by which HbXIP2;1 ensures glycerol transfer across the membrane. From these results, we discuss the physiological involvement of glycerol and HbXIP2;1 in water homeostasis and carbon stream of challenged laticifers. The characterization of HbXIP2;1 during rubber tree tapping lends new insights into molecular and physiological response processes of laticifer metabolism in the context of latex exploitation. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 38% |
| Student > Bachelor | 2 | 13% |
| Student > Ph. D. Student | 2 | 13% |
| Professor > Associate Professor | 2 | 13% |
| Student > Master | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 2 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 56% |
| Biochemistry, Genetics and Molecular Biology | 3 | 19% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
| Unknown | 3 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 April 2016.
All research outputs
#17,797,589
of 22,862,742 outputs
Outputs from Plant Molecular Biology
#2,449
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Outputs of similar age
#206,478
of 300,949 outputs
Outputs of similar age from Plant Molecular Biology
#11
of 24 outputs
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