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Redox-signaling transmitted in trans to neighboring cells by melanoma-derived TNF-containing exosomes

Overview of attention for article published in Free Radical Biology & Medicine, March 2007
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Title
Redox-signaling transmitted in trans to neighboring cells by melanoma-derived TNF-containing exosomes
Published in
Free Radical Biology & Medicine, March 2007
DOI 10.1016/j.freeradbiomed.2007.03.026
Pubmed ID
Authors

Anita Söderberg, Ana María Barral, Mats Söderström, Birgitta Sander, Anders Rosén

Abstract

Hydrogen peroxide is known to be involved in redox signaling pathways that regulate normal processes and disease progression, including cytokine signaling, oxidative stress, and cancer. In studies on immune surveillance against cancer, hydrogen peroxide was found to disrupt cytotoxic T-cell function, thus contributing to tumor escape. In this study, secretion of TNF-containing vesicles of rab9+ endosomal origin, termed exosomes, was investigated using GFP-TNF constructs. We observed a polarized intracellular trafficking and apical secretion of TNF-positive nanovesicles. Cell-to-cell transfer of TNF was observed in exosomes in real-time microscopy, occurring separate from the melanin/melanosome compartment. Exosomes were prepared by ultracentrifugation or immunoisolation on anti-beta2-microglobulin magnetic beads. TNF as well as TNF receptors 1 and 2 were present in the exosomes as determined by Western blot, flow cytometry, and deconvolution microscopy. The functional significance of melanoma-derived exosomes was established by their signaling competence with ability to generate significantly higher ROS levels in T cells compared with sham exosomes (P=0.0006). In conclusion, we report here, for the first time, that TNF is found in tumor cell-derived exosomes and that these exosomes transmit redox signaling in trans to neighboring cells. The results are of importance for a better understanding of tumor escape mechanisms.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 80 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 1%
China 1 1%
Argentina 1 1%
Unknown 77 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 22 28%
Researcher 19 24%
Professor > Associate Professor 7 9%
Student > Master 6 8%
Professor 5 6%
Other 11 14%
Unknown 10 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 24 30%
Biochemistry, Genetics and Molecular Biology 15 19%
Medicine and Dentistry 11 14%
Immunology and Microbiology 6 8%
Pharmacology, Toxicology and Pharmaceutical Science 4 5%
Other 7 9%
Unknown 13 16%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 December 2012.
All research outputs
#7,786,691
of 25,394,764 outputs
Outputs from Free Radical Biology & Medicine
#1,691
of 5,449 outputs
Outputs of similar age
#30,715
of 91,632 outputs
Outputs of similar age from Free Radical Biology & Medicine
#27
of 51 outputs
Altmetric has tracked 25,394,764 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 5,449 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.8. This one has gotten more attention than average, scoring higher than 68% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 91,632 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 51 others from the same source and published within six weeks on either side of this one. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.