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Mendeley readers
Attention Score in Context
| Title |
Analysis of the function of IL-10 in chickens using specific neutralising antibodies and a sensitive capture ELISA
|
|---|---|
| Published in |
Developmental & Comparative Immunology, April 2016
|
| DOI | 10.1016/j.dci.2016.04.016 |
| Pubmed ID | |
| Authors |
Zhiguang Wu, Tuanjun Hu, Lisa Rothwell, Lonneke Vervelde, Pete Kaiser, Kay Boulton, Matthew J. Nolan, Fiona M. Tomley, Damer P. Blake, David A. Hume |
| Abstract |
In mammals, the inducible cytokine interleukin 10 is a feedback negative regulator of inflammation. To determine the extent to which this function is conserved in birds, recombinant chicken IL-10 was expressed as a secreted human Ig Fc fusion protein (chIL-10-Fc) and used to immunise mice. Five monoclonal antibodies (mAb) which specifically recognise chicken IL-10 were generated and characterised. Two capture ELISA assays were developed which detected native chIL-10 secreted from chicken bone marrow-derived macrophages (chBMMs) stimulated with lipopolysaccharide (LPS). Three of the mAbs detected intracellular IL-10. This was detected in only a subset of the same LPS-stimulated chBMMs. The ELISA assay also detected massive increases in circulating IL-10 in chickens challenged with the coccidial parasite, E. tenella, and the level correlated with the lesion score. The same mAbs neutralised the bioactivity of recombinant chIL-10. The role of IL-10 in feedback control was tested in vitro. The neutralising antibodies prevented IL-10-induced inhibition of IFN-γ synthesis by mitogen-activated lymphocytes and increased nitric oxide production in LPS-stimulated chBMMs. The results confirm that IL-10 is an inducible feedback regulator of immune response in chickens, and could be the target for improved vaccine efficacy or breeding strategies. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 76 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 1% |
| Unknown | 75 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 19 | 25% |
| Student > Master | 12 | 16% |
| Researcher | 9 | 12% |
| Student > Bachelor | 4 | 5% |
| Student > Doctoral Student | 3 | 4% |
| Other | 8 | 11% |
| Unknown | 21 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 24 | 32% |
| Veterinary Science and Veterinary Medicine | 9 | 12% |
| Biochemistry, Genetics and Molecular Biology | 9 | 12% |
| Immunology and Microbiology | 4 | 5% |
| Engineering | 2 | 3% |
| Other | 5 | 7% |
| Unknown | 23 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 November 2017.
All research outputs
#19,942,887
of 25,371,288 outputs
Outputs from Developmental & Comparative Immunology
#966
of 1,841 outputs
Outputs of similar age
#218,362
of 313,297 outputs
Outputs of similar age from Developmental & Comparative Immunology
#17
of 47 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one is in the 18th percentile – i.e., 18% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,841 research outputs from this source. They receive a mean Attention Score of 2.1. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 313,297 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 47 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.