Title |
Remodeling the zonula adherens in response to tension and the role of afadin in this response
|
---|---|
Published in |
Journal of Cell Biology, April 2016
|
DOI | 10.1083/jcb.201506115 |
Pubmed ID | |
Authors |
Wangsun Choi, Bipul R. Acharya, Grégoire Peyret, Marc-Antoine Fardin, René-Marc Mège, Benoit Ladoux, Alpha S. Yap, Alan S. Fanning, Mark Peifer |
Abstract |
Morphogenesis requires dynamic coordination between cell-cell adhesion and the cytoskeleton to allow cells to change shape and move without losing tissue integrity. We used genetic tools and superresolution microscopy in a simple model epithelial cell line to define how the molecular architecture of cell-cell zonula adherens (ZA) is modified in response to elevated contractility, and how these cells maintain tissue integrity. We previously found that depleting zonula occludens 1 (ZO-1) family proteins in MDCK cells induces a highly organized contractile actomyosin array at the ZA. We find that ZO knockdown elevates contractility via a Shroom3/Rho-associated, coiled-coil containing protein kinase (ROCK) pathway. Our data suggest that each bicellular border is an independent contractile unit, with actin cables anchored end-on to cadherin complexes at tricellular junctions. Cells respond to elevated contractility by increasing junctional afadin. Although ZO/afadin knockdown did not prevent contractile array assembly, it dramatically altered cell shape and barrier function in response to elevated contractility. We propose that afadin acts as a robust protein scaffold that maintains ZA architecture at tricellular junctions. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 50% |
Unknown | 2 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 75% |
Science communicators (journalists, bloggers, editors) | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Japan | 1 | <1% |
United States | 1 | <1% |
Germany | 1 | <1% |
Unknown | 145 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 41 | 28% |
Researcher | 28 | 19% |
Student > Bachelor | 14 | 9% |
Student > Master | 10 | 7% |
Other | 7 | 5% |
Other | 17 | 11% |
Unknown | 31 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 52 | 35% |
Agricultural and Biological Sciences | 44 | 30% |
Physics and Astronomy | 6 | 4% |
Medicine and Dentistry | 3 | 2% |
Unspecified | 2 | 1% |
Other | 8 | 5% |
Unknown | 33 | 22% |