Title |
RhoC GTPase Is a Potent Regulator of Glutamine Metabolism and N-Acetylaspartate Production in Inflammatory Breast Cancer Cells*
|
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Published in |
Journal of Biological Chemistry, April 2016
|
DOI | 10.1074/jbc.m115.703959 |
Pubmed ID | |
Authors |
Michelle L Wynn, Joel A Yates, Charles R Evans, Lauren D Van Wassenhove, Zhi Fen Wu, Sydney Bridges, Liwei Bao, Chelsea Fournier, Sepideh Ashrafzadeh, Matthew J Merrins, Leslie S Satin, Santiago Schnell, Charles F Burant, Sofia D Merajver |
Abstract |
Inflammatory breast cancer (IBC) is an extremely lethal cancer that rapidly metastasizes. While the molecular attributes of IBC have been described, little is known about the underlying metabolic features of the disease. Using a variety of metabolic assays including (13)C tracer experiments, we found that SUM149 cells, the primary in vitro model of IBC, exhibit metabolic abnormalities that distinguish them from other breast cancer cells, including elevated levels of N-acetylaspartate (NAA), a metabolite primarily associated with neuronal disorders and gliomas. Here we provide the first evidence of NAA in breast cancer. We also report that the oncogene RhoC, a driver of metastatic potential, modulates glutamine and N-acetylaspartate metabolism in IBC cells in vitro, revealing a novel role for RhoC as a regulator of tumor cell metabolism that extends beyond its well-known role in cytoskeletal rearrangement. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 6 | 67% |
Spain | 1 | 11% |
Unknown | 2 | 22% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 6 | 67% |
Scientists | 3 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 2% |
Unknown | 45 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 13 | 28% |
Student > Master | 6 | 13% |
Researcher | 4 | 9% |
Student > Postgraduate | 3 | 7% |
Student > Bachelor | 2 | 4% |
Other | 3 | 7% |
Unknown | 15 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 12 | 26% |
Agricultural and Biological Sciences | 9 | 20% |
Chemistry | 2 | 4% |
Medicine and Dentistry | 2 | 4% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
Other | 5 | 11% |
Unknown | 15 | 33% |