| Title |
Production of hydrophobic amino acids from biobased resources: wheat gluten and rubber seed proteins
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, April 2016
|
| DOI | 10.1007/s00253-016-7441-8 |
| Pubmed ID | |
| Authors |
Widyarani, Yessie W. Sari, Enny Ratnaningsih, Johan P. M. Sanders, Marieke E. Bruins |
| Abstract |
Protein hydrolysis enables production of peptides and free amino acids that are suitable for usage in food and feed or can be used as precursors for bulk chemicals. Several essential amino acids for food and feed have hydrophobic side chains; this property may also be exploited for subsequent separation. Here, we present methods for selective production of hydrophobic amino acids from proteins. Selectivity can be achieved by selection of starting material, selection of hydrolysis conditions, and separation of achieved hydrolysate. Several protease combinations were applied for hydrolysis of rubber seed protein concentrate, wheat gluten, and bovine serum albumin (BSA). High degree of hydrolysis (>50 %) could be achieved. Hydrophobic selectivity was influenced by the combination of proteases and by the extent of hydrolysis. Combination of Pronase and Peptidase R showed the highest selectivity towards hydrophobic amino acids, roughly doubling the content of hydrophobic amino acids in the products compared to the original substrates. Hydrophobic selectivity of 0.6 mol-hydrophobic/mol-total free amino acids was observed after 6 h hydrolysis of wheat gluten and 24 h hydrolysis of rubber seed proteins and BSA. The results of experiments with rubber seed proteins and wheat gluten suggest that this process can be applied to agro-industrial residues. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 72 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 72 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 14% |
| Student > Master | 8 | 11% |
| Researcher | 7 | 10% |
| Lecturer | 4 | 6% |
| Professor | 4 | 6% |
| Other | 12 | 17% |
| Unknown | 27 | 38% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 15 | 21% |
| Biochemistry, Genetics and Molecular Biology | 9 | 13% |
| Chemistry | 4 | 6% |
| Materials Science | 3 | 4% |
| Unspecified | 2 | 3% |
| Other | 12 | 17% |
| Unknown | 27 | 38% |
Attention Score in Context
This research output has an Altmetric Attention Score of 17. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 December 2023.
All research outputs
#2,118,005
of 24,942,536 outputs
Outputs from Applied Microbiology and Biotechnology
#148
of 8,167 outputs
Outputs of similar age
#33,682
of 304,872 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#6
of 133 outputs
Altmetric has tracked 24,942,536 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 91st percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,167 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done particularly well, scoring higher than 98% of its peers.
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We're also able to compare this research output to 133 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.