| Title |
Abnormal overexpression of mastocytes in skin biopsies of fibromyalgia patients
|
|---|---|
| Published in |
Clinical Rheumatology, April 2010
|
| DOI | 10.1007/s10067-010-1474-7 |
| Pubmed ID | |
| Authors |
Ignacio Blanco, Nana Béritze, Mario Argüelles, Victoriano Cárcaba, Fernando Fernández, Sabina Janciauskiene, Katerina Oikonomopoulou, Frederick J. de Serres, Enrique Fernández-Bustillo, Morley D. Hollenberg |
| Abstract |
Formalin-fixed, paraffin-embedded skin tissue sections were collected from a matched cohort of 63 fibromyalgia syndrome (FMS) patients and 49 volunteers from the general population with both alpha1-antitrypsin (AAT) normal and deficiency variants. These tissues were examined for the expression of the broad-spectrum inhibitor AAT, the serine proteinases elastase and tryptase, the proinflammatory cytokines MCP-1 and TNFα, the endothelium biomarker VEGF, and the inflammation/nociception-related receptor PAR(2). The most relevant finding of the study was a significantly increased number of mast cells (MCs) in the papillary dermis of all FMS patients (greater than or equal to five to 14 per microscopic high power field) compared to zero to one in controls (p < 0.001). MCs strongly stained with tryptase, AAT and PAR(2) antibodies, exhibited a spindle-like shape and were uniformly distributed around blood vessels and appendages. MCP-1 and VEGF expressed weak/moderate positivity in most samples, with a higher expression in controls than in FMS patients (p < 0.001 and 0.051, respectively). No differences in elastase and TNFα were found between both groups. Moreover, no histological differences were found between samples from AAT deficiency and normal AAT phenotypes. Our results indicate that FMS is a MC-associated condition. MCs are present in skin and mucosal surfaces throughout the human body, and are easily stimulated by a number of physical, psychological, and chemical triggers to degranulate, releasing several proinflammatory products which are able to generate nervous peripheral stimuli causing CNS hypersensitivity, local, and systemic symptoms. Our findings open new avenues of research on FMS mechanisms and will benefit the diagnosis of patients and the development of therapeutics. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 17% |
| United States | 1 | 17% |
| Spain | 1 | 17% |
| Unknown | 3 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 6 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 94 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 1% |
| Mexico | 1 | 1% |
| Unknown | 92 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 20 | 21% |
| Student > Bachelor | 15 | 16% |
| Researcher | 10 | 11% |
| Other | 6 | 6% |
| Student > Postgraduate | 6 | 6% |
| Other | 18 | 19% |
| Unknown | 19 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 22 | 23% |
| Agricultural and Biological Sciences | 17 | 18% |
| Nursing and Health Professions | 9 | 10% |
| Biochemistry, Genetics and Molecular Biology | 7 | 7% |
| Psychology | 5 | 5% |
| Other | 14 | 15% |
| Unknown | 20 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 January 2020.
All research outputs
#4,484,826
of 22,664,267 outputs
Outputs from Clinical Rheumatology
#705
of 2,977 outputs
Outputs of similar age
#19,108
of 94,694 outputs
Outputs of similar age from Clinical Rheumatology
#2
of 12 outputs
Altmetric has tracked 22,664,267 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,977 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.9. This one has done well, scoring higher than 76% of its peers.
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We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 83% of its contemporaries.