| Title |
The aromatic peroxygenase from Marasmius rutola—a new enzyme for biosensor applications
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, October 2011
|
| DOI | 10.1007/s00216-011-5497-y |
| Pubmed ID | |
| Authors |
Aysu Yarman, Glenn Gröbe, Bettina Neumann, Mathias Kinne, Nenad Gajovic-Eichelmann, Ulla Wollenberger, Martin Hofrichter, René Ullrich, Katrin Scheibner, Frieder W. Scheller |
| Abstract |
The aromatic peroxygenase (APO; EC 1.11.2.1) from the agraric basidomycete Marasmius rotula (MroAPO) immobilized at the chitosan-capped gold-nanoparticle-modified glassy carbon electrode displayed a pair of redox peaks with a midpoint potential of -278.5 mV vs. AgCl/AgCl (1 M KCl) for the Fe(2+)/Fe(3+) redox couple of the heme-thiolate-containing protein. MroAPO oxidizes aromatic substrates such as aniline, p-aminophenol, hydroquinone, resorcinol, catechol, and paracetamol by means of hydrogen peroxide. The substrate spectrum overlaps with those of cytochrome P450s and plant peroxidases which are relevant in environmental analysis and drug monitoring. In M. rotula peroxygenase-based enzyme electrodes, the signal is generated by the reduction of electrode-active reaction products (e.g., p-benzoquinone and p-quinoneimine) with electro-enzymatic recycling of the analyte. In these enzyme electrodes, the signal reflects the conversion of all substrates thus representing an overall parameter in complex media. The performance of these sensors and their further development are discussed. |
Mendeley readers
The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 3% |
| Unknown | 39 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 7 | 18% |
| Student > Postgraduate | 6 | 15% |
| Researcher | 6 | 15% |
| Student > Ph. D. Student | 5 | 13% |
| Professor | 3 | 8% |
| Other | 4 | 10% |
| Unknown | 9 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 10 | 25% |
| Biochemistry, Genetics and Molecular Biology | 10 | 25% |
| Chemistry | 3 | 8% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 5% |
| Social Sciences | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 14 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 July 2015.
All research outputs
#8,681,963
of 25,728,855 outputs
Outputs from Analytical & Bioanalytical Chemistry
#2,254
of 9,730 outputs
Outputs of similar age
#52,287
of 153,295 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#28
of 74 outputs
Altmetric has tracked 25,728,855 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 9,730 research outputs from this source. They receive a mean Attention Score of 3.1. This one has gotten more attention than average, scoring higher than 55% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 153,295 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 39th percentile – i.e., 39% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 74 others from the same source and published within six weeks on either side of this one. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.