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Characterisation of a mouse cerebral microvascular endothelial cell line (bEnd.3) after oxygen glucose deprivation and reoxygenation

Overview of attention for article published in Clinical & Experimental Pharmacology & Physiology, July 2016
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#35 of 1,426)
  • High Attention Score compared to outputs of the same age (90th percentile)
  • High Attention Score compared to outputs of the same age and source (93rd percentile)

Mentioned by

news
1 news outlet
blogs
1 blog
twitter
1 X user
facebook
1 Facebook page
wikipedia
2 Wikipedia pages

Citations

dimensions_citation
21 Dimensions

Readers on

mendeley
45 Mendeley
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Title
Characterisation of a mouse cerebral microvascular endothelial cell line (bEnd.3) after oxygen glucose deprivation and reoxygenation
Published in
Clinical & Experimental Pharmacology & Physiology, July 2016
DOI 10.1111/1440-1681.12587
Pubmed ID
Authors

Jacqueline M Ku, Mohammadali Taher, Kai Yee Chin, Megan Grace, Peter McIntyre, Alyson A Miller

Abstract

Studies have utilised immortalised mouse cerebral endothelial cells (bEnd.3) exposed to oxygen glucose deprivation (OGD) to study blood-brain barrier (BBB) disruption after ischaemia. However, there is a paucity of literature describing the duration of OGD (and reoxygenation [RO]) required to best simulate BBB disruption in vivo. In this study we assessed BBB disruption in bEnd.3 cells after exposure to a range of OGD periods, and also after OGD + RO. Exposure of bEnd.3 monolayers to 4, 6, 16, or 24 h of OGD resulted in a significant increase in permeability. The hyperpermeability after 16 or 24 h was associated with decreased expression of tight junction proteins (occludin and claudin-5). Furthermore, there was a decrease in cell viability and increased expression of the pro-apoptotic protein, cleaved caspase-3. Exposure of bEnd.3 monolayers to 1 h OGD + 23 h RO exacerbated hyperpermeability relative to 1 h OGD, which was associated with decreased expression levels of occludin and ZO-1, but no change in cell viability or caspase-3. 4 h OGD + 23 h RO exacerbated hyperpermeability, decreased expression levels of tight junction proteins, decreased cell viability, and increased caspase-3 expression. Thus, bEnd.3 cells exhibit hyperpermeability, a loss of tight junction proteins, and undergo cell death, after exposure to prolonged periods of OGD. Moreover, they exhibit exacerbated hyperpermeability, a loss of tight junction proteins, and increased expression of caspase-3 after OGD + RO. These findings will facilitate the use of this cell line in studies of BBB disruption and for the testing of therapeutics. This article is protected by copyright. All rights reserved.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 2%
Unknown 44 98%

Demographic breakdown

Readers by professional status Count As %
Student > Master 10 22%
Student > Ph. D. Student 9 20%
Researcher 6 13%
Student > Bachelor 6 13%
Student > Doctoral Student 3 7%
Other 2 4%
Unknown 9 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 20%
Agricultural and Biological Sciences 7 16%
Medicine and Dentistry 5 11%
Neuroscience 5 11%
Chemistry 2 4%
Other 7 16%
Unknown 10 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 18. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 November 2019.
All research outputs
#2,033,600
of 25,373,627 outputs
Outputs from Clinical & Experimental Pharmacology & Physiology
#35
of 1,426 outputs
Outputs of similar age
#36,097
of 369,846 outputs
Outputs of similar age from Clinical & Experimental Pharmacology & Physiology
#1
of 15 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 91st percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,426 research outputs from this source. They receive a mean Attention Score of 4.2. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 369,846 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 15 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.