| Title |
Antipsychotic Induced Alteration of Growth and Proteome of Rat Neural Stem Cells
|
|---|---|
| Published in |
Neurochemical Research, April 2012
|
| DOI | 10.1007/s11064-012-0768-3 |
| Pubmed ID | |
| Authors |
Eakhlas Uddin Ahmed, Selina Ahmed, Wataru Ukai, Izuru Matsumoto, Andrew Kemp, Iain S. McGregor, Mohammed Abul Kashem |
| Abstract |
Neural stem cells (NSCs) play a crucial role in the development and maturation of the central nervous system and therefore have the potential to target by therapeutic agents for a wide variety of diseases including neurodegenerative and neuropsychiatric illnesses. It has been suggested that antipsychotic drugs have significant effects on NSC activities. However, the molecular mechanisms underlying antipsychotic-induced changes of NSC activities, particularly growth and protein expression, are largely unknown. NSCs were treated with either haloperidol (HD; 3 μM), risperidone (RS; 3 μM) or vehicle (DMSO) for 96 h. Protein expression profiles were studied through a proteomics approach. RS promoted and HD inhibited the growth of NSCs. Proteomics analysis revealed that 15 protein spots identified as 12 unique proteins in HD-, and 20 protein spots identified as 14 proteins in RS-treated groups, were differentially expressed relative to control. When these identified proteins were compared between the two drug-treated groups, 2 proteins overlapped leaving 10 HD-specific and 12 RS-specific proteins. Further comparison of the overlapped altered proteins of 96 h treatment with the neuroleptics-induced overlapped proteins at 24 h time interval (Kashem et al. [40] in Neurochem Int 55:558-565, 2009) suggested that overlapping altered proteins expression at 24 h was decreased (17 proteins i.e. 53 % of total expressed proteins) with the increase of time (96 h) (2 proteins; 8 % of total expressed proteins). This result indicated that at early stage both drugs showed common mode of action but the action was opposite to each other while administration was prolonged. The opposite morphological pattern of cellular growth at 96 h has been associated with dominant expression of oxidative stress and apoptosis cascades in HD, and activation of growth regulating metabolic pathways in RS treated cells. These results may explain RS induced repairing of neural damage caused by a wide variety of neural diseases including schizophrenia. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Austria | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 4% |
| Unknown | 22 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 5 | 22% |
| Student > Ph. D. Student | 5 | 22% |
| Professor > Associate Professor | 4 | 17% |
| Researcher | 4 | 17% |
| Student > Doctoral Student | 3 | 13% |
| Other | 2 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 30% |
| Biochemistry, Genetics and Molecular Biology | 5 | 22% |
| Medicine and Dentistry | 4 | 17% |
| Neuroscience | 3 | 13% |
| Psychology | 2 | 9% |
| Other | 2 | 9% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 April 2012.
All research outputs
#20,880,816
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Outputs from Neurochemical Research
#1,649
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Outputs of similar age
#136,823
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Outputs of similar age from Neurochemical Research
#18
of 25 outputs
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