| Title |
d-Aspartate drinking solution alleviates pain and cognitive impairment in neuropathic mice
|
|---|---|
| Published in |
Amino Acids, April 2016
|
| DOI | 10.1007/s00726-016-2205-4 |
| Pubmed ID | |
| Authors |
Enza Palazzo, Livio Luongo, Francesca Guida, Ida Marabese, Rosaria Romano, Monica Iannotta, Francesca Rossi, Antimo D’Aniello, Luigi Stella, Federica Marmo, Alessandro Usiello, Andrea de Bartolomeis, Sabatino Maione, Vito de Novellis |
| Abstract |
D-Aspartate (D-Asp) is a free D-amino acid detected in multiple brain regions and putative precursor of endogenous N-methyl-D-aspartate (NMDA) acting as agonist at NMDA receptors. In this study, we investigated whether D-Asp (20 mM) in drinking solution for 1 month affects pain responses and pain-related emotional, and cognitive behaviour in a model of neuropathic pain induced by the spared nerve injury (SNI) of the sciatic nerve in mice. SNI mice developed mechanical allodynia and motor coordination impairment 30 days after SNI surgery. SNI mice showed cognitive impairment, anxiety and depression-like behaviour, reduced sociability in the three chamber sociability paradigm, increased expression of NR2B subunit of NMDA receptor and Homer 1a in the medial prefrontal cortex (mPFC). The expression of (post synaptic density) PSD-95 and Shank 1was instead unaffected in the mPFC of the SNI mice. Treatment with D-Asp drinking solution, started right after the SNI (day 0), alleviated mechanical allodynia, improved cognition and motor coordination and increased social interaction. D-Asp also restored the levels of extracellular D-Asp, Homer 1a and NR2B subunit of the NMDA receptor to physiological levels and reduced Shank1 and PSD-95 protein levels in the mPFC. Amitriptyline, a tricyclic antidepressant used also to alleviate neuropathic pain in humans, reverted mechanical allodynia and cognitive impairment, and unlike D-Asp, was effective in reducing depression and anxiety-like behaviour in the SNI mice and increased PSD protein level. Altogether these findings demonstrate that D-Asp improves sensorial, motor and cognitive-like symptoms related to chronic pain possibly through glutamate neurotransmission normalization in neuropathic mice. |
Mendeley readers
The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 1% |
| Unknown | 66 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 12 | 18% |
| Student > Ph. D. Student | 11 | 16% |
| Researcher | 9 | 13% |
| Student > Bachelor | 4 | 6% |
| Professor > Associate Professor | 4 | 6% |
| Other | 6 | 9% |
| Unknown | 21 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 12 | 18% |
| Medicine and Dentistry | 6 | 9% |
| Biochemistry, Genetics and Molecular Biology | 5 | 7% |
| Agricultural and Biological Sciences | 5 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 5 | 7% |
| Other | 11 | 16% |
| Unknown | 23 | 34% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 May 2016.
All research outputs
#20,323,943
of 22,867,327 outputs
Outputs from Amino Acids
#1,285
of 1,520 outputs
Outputs of similar age
#253,209
of 298,924 outputs
Outputs of similar age from Amino Acids
#29
of 34 outputs
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