| Title |
Identification and Characterization of a δ-Cadinol Synthase Potentially Involved in the Formation of Boreovibrins in Boreostereum vibrans of Basidiomycota
|
|---|---|
| Published in |
Natural Products and Bioprospecting, April 2016
|
| DOI | 10.1007/s13659-016-0096-4 |
| Pubmed ID | |
| Authors |
Hui Zhou, Yan-Long Yang, Jun Zeng, Ling Zhang, Zhi-Hui Ding, Ying Zeng |
| Abstract |
Sesquiterpenoids are very common among natural products. A large number of sesquiterpene synthase genes have been cloned and functionally characterized. However, until now there is no report about the δ-cadinol synthase predominantly forming δ-cadinol (syn. torreyol) from farnesyl diphosphate. Sesquiterpenoids boreovibrins structurally similar to δ-cadinol were previously isolated from culture broths of the basidiomycete fungus Boreostereum vibrans. This led us to expect a corresponding gene coding for a δ-cadinol synthase that may be involved in the biosynthesis of boreovibrins in B. vibrans. Here we report the cloning and heterologous expression of a new sesquiterpene synthase gene from B. vibrans. The crude and purified recombinant enzymes, when incubating with farnesyl diphosphate as substrate, gave δ-cadinol as its principal product and thereby identified as a δ-cadinol synthase. A new sesquiterpene synthase gene was cloned from the basidiomycete fungus Boreostereum vibrans and heterologously expressed in E. coli. The purified recombinant enzyme gave δ-cadinol as its principal product from farnesyl diphosphate and thereby identified as a δ-cadinol synthase (BvCS). |
Mendeley readers
The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 5% |
| Unknown | 18 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 21% |
| Student > Doctoral Student | 3 | 16% |
| Researcher | 3 | 16% |
| Student > Master | 3 | 16% |
| Professor > Associate Professor | 2 | 11% |
| Other | 2 | 11% |
| Unknown | 2 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 37% |
| Biochemistry, Genetics and Molecular Biology | 5 | 26% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
| Nursing and Health Professions | 1 | 5% |
| Chemistry | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 4 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 May 2016.
All research outputs
#4,188,738
of 22,867,327 outputs
Outputs from Natural Products and Bioprospecting
#16
of 132 outputs
Outputs of similar age
#66,108
of 300,274 outputs
Outputs of similar age from Natural Products and Bioprospecting
#1
of 3 outputs
Altmetric has tracked 22,867,327 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 132 research outputs from this source. They receive a mean Attention Score of 4.4. This one has done well, scoring higher than 82% of its peers.
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We're also able to compare this research output to 3 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them