| Title |
Improvement of thermostability and activity of Trichoderma reesei endo-xylanase Xyn III on insoluble substrates
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, April 2016
|
| DOI | 10.1007/s00253-016-7563-z |
| Pubmed ID | |
| Authors |
Tomohiko Matsuzawa, Satoshi Kaneko, Katsuro Yaoi |
| Abstract |
Trichoderma reesei Xyn III, an endo-β-1,4-xylanase belonging to glycoside hydrolase family 10 (GH10), is vital for the saccharification of xylans in plant biomass. However, its enzymatic thermostability and hydrolytic activity on insoluble substrates are low. To overcome these difficulties, the thermostability of Xyn III was improved using random mutagenesis and directed evolution, and its hydrolytic activity on insoluble substrates was improved by creating a chimeric protein. In the screening of thermostable Xyn III mutants from a random mutagenesis library, we identified two amino acid residues, Gln286 and Asn340, which are important for the thermostability of Xyn III. The Xyn III Gln286Ala/Asn340Tyr mutant showed xylanase activity even after heat treatment at 60 °C for 30 min or 50 °C for 96 h, indicating a dramatic enhancement in thermostability. In addition, we found that the addition of a xylan-binding domain (XBD) to the C-terminal of Xyn III improved its hydrolytic activity on insoluble xylan. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| China | 1 | 4% |
| Unknown | 22 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 4 | 17% |
| Student > Ph. D. Student | 4 | 17% |
| Researcher | 4 | 17% |
| Student > Bachelor | 3 | 13% |
| Student > Master | 3 | 13% |
| Other | 2 | 9% |
| Unknown | 3 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 35% |
| Agricultural and Biological Sciences | 8 | 35% |
| Engineering | 2 | 9% |
| Medicine and Dentistry | 1 | 4% |
| Unknown | 4 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 May 2016.
All research outputs
#19,611,252
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#6,478
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Outputs of similar age
#224,126
of 302,743 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#95
of 130 outputs
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