| Title |
Upregulation of the clpB gene in response to heat shock and beta-lactam antibiotics in Acinetobacter baumannii
|
|---|---|
| Published in |
Molecular Biology Reports, November 2019
|
| DOI | 10.1007/s11033-019-05209-4 |
| Pubmed ID | |
| Authors |
Waleska Yana Lazaretti, Elaine Luzia dos Santos, José Luis da-Conceição Silva, Marina Kimiko Kadowaki, Rinaldo Ferreira Gandra, Alexandre Maller, Rita de Cássia Garcia Simão |
| Abstract |
The role of the clpB gene encoding HSP/chaperone ClpB was evaluated in the multiresistant antibiotic cells of Acinetobacter baumannii (RS4 strain) under stress-induced heat shock and different beta-lactams. The expression of the clpB gene was assessed by qPCR during heat shock at 45 °C and subinhibitory concentrations of ampicillin (30 μg mL-1), amoxicillin + sulbactam (8/12 μg mL-1), cefepime (30 μg mL-1), sulfamethoxazole + trimethoprim (120/8 μg mL-1) and meropenem (18 μg mL-1). The results indicated a transient increase in clpB transcription in all treatments except cefepime. Both in the presence of ampicillin and amoxicillin/sulbactam for 20 min, the mRNA-clpB synthesis was 1.4 times higher than that of the control at time zero. Surprisingly, the mRNA-clpB levels were more than 30-fold higher after 10 min of incubation with meropenem and more than eightfold higher in the presence of trimethoprim/sulfamethoxazole. In addition, western blot assays showed that the RS4 strain treated with meropenem showed a marked increase in ClpB protein expression. Our data indicate that during exposure to beta-lactams, A. baumannii adjusts the transcription levels of the clpB mRNA and protein to respond to stress, suggesting that the chaperone may act as a key cellular component in the presence of antibiotics in this bacterium. |
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 3 | 14% |
| Student > Doctoral Student | 2 | 10% |
| Student > Bachelor | 2 | 10% |
| Professor > Associate Professor | 2 | 10% |
| Other | 1 | 5% |
| Other | 4 | 19% |
| Unknown | 7 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 43% |
| Agricultural and Biological Sciences | 2 | 10% |
| Environmental Science | 1 | 5% |
| Medicine and Dentistry | 1 | 5% |
| Engineering | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 7 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 December 2019.
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#20,592,137
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Outputs from Molecular Biology Reports
#2,076
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#384,732
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Outputs of similar age from Molecular Biology Reports
#41
of 63 outputs
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