Title |
Utilization of Paneer Whey Waste for Cost-Effective Production of Rhamnolipid Biosurfactant
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Published in |
Applied Biochemistry and Biotechnology, May 2016
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DOI | 10.1007/s12010-016-2105-9 |
Pubmed ID | |
Authors |
Rupshikha Patowary, Kaustuvmani Patowary, Mohan Chandra Kalita, Suresh Deka |
Abstract |
The present study aimed at isolating rhamnolipid biosurfactant-producing bacteria that could utilize paneer whey, an abundant waste source as sole medium for the production purpose. Pseudomonas aeruginosa strain, SR17, was isolated from hydrocarbon-contaminated soil that could efficiently utilize paneer whey for rhamnolipid production and reduce surface tension of the medium from 52 to 26.5 mN/m. The yield of biosurfactant obtained was 2.7 g/l, upgraded to 4.8 g/l when supplemented with 2 % glucose and mineral salts. Biochemical, FTIR, and LC-MS analysis revealed that extracted biosurfactant is a combination of both mono and di-rhamnolipid congeners. The critical micelle concentration (CMC) was measured to be 110 mg/l. Emulsification activity of the biosurfactant against n-hexadecane, olive oil, kerosene, diesel oil, engine oil, and crude oil were found to be 83, 88, 81, 92, 86, and 100 %, respectively. The rhamnolipid was detected to be non-toxic against mouse fibroblastic cell line L292. |
X Demographics
Geographical breakdown
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 92 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 12 | 13% |
Student > Bachelor | 11 | 12% |
Student > Ph. D. Student | 9 | 10% |
Researcher | 8 | 9% |
Professor > Associate Professor | 5 | 5% |
Other | 11 | 12% |
Unknown | 36 | 39% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 13 | 14% |
Biochemistry, Genetics and Molecular Biology | 9 | 10% |
Immunology and Microbiology | 7 | 8% |
Environmental Science | 5 | 5% |
Engineering | 5 | 5% |
Other | 10 | 11% |
Unknown | 43 | 47% |