↓ Skip to main content

Alleviating monoterpene toxicity using a two‐phase extractive fermentation for the bioproduction of jet fuel mixtures in Saccharomyces cerevisiae

Overview of attention for article published in Biotechnology & Bioengineering, May 2012
Altmetric Badge

About this Attention Score

  • Good Attention Score compared to outputs of the same age (67th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (57th percentile)

Mentioned by

twitter
1 X user
patent
3 patents

Citations

dimensions_citation
159 Dimensions

Readers on

mendeley
263 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Alleviating monoterpene toxicity using a two‐phase extractive fermentation for the bioproduction of jet fuel mixtures in Saccharomyces cerevisiae
Published in
Biotechnology & Bioengineering, May 2012
DOI 10.1002/bit.24536
Pubmed ID
Authors

Timothy C.R. Brennan, Christopher D. Turner, Jens O. Krömer, Lars K. Nielsen

Abstract

Monoterpenes are a diverse class of compounds with applications as flavors and fragrances, pharmaceuticals and more recently, jet fuels. Engineering biosynthetic pathways for monoterpene production in microbial hosts has received increasing attention. However, monoterpenes are highly toxic to many microorganisms including Saccharomyces cerevisiae, a widely used industrial biocatalyst. In this work, the minimum inhibitory concentration (MIC) for S. cerevisiae was determined for five monoterpenes: β-pinene, limonene, myrcene, γ-terpinene, and terpinolene (1.52, 0.44, 2.12, 0.70, 0.53 mM, respectively). Given the low MIC for all compounds tested, a liquid two-phase solvent extraction system to alleviate toxicity during fermentation was evaluated. Ten solvents were tested for biocompatibility, monoterpene distribution, phase separation, and price. The solvents dioctyl phthalate, dibutyl phthalate, isopropyl myristate, and farnesene showed greater than 100-fold increase in the MIC compared to the monoterpenes in a solvent-free system. In particular, the MIC for limonene in dibutyl phthalate showed a 702-fold (308 mM, 42.1 g L(-1) of limonene) improvement while cell viability was maintained above 90%, demonstrating that extractive fermentation is a suitable tool for the reduction of monoterpene toxicity. Finally, we estimated that a limonane to farnesane ratio of 1:9 has physicochemical properties similar to traditional Jet-A aviation fuel. Since farnesene is currently produced in S. cerevisiae, its use as a co-product and extractant for microbial terpene-based jet fuel production in a two-phase system offers an attractive bioprocessing option.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 263 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 6 2%
Canada 2 <1%
Chile 1 <1%
Austria 1 <1%
Switzerland 1 <1%
India 1 <1%
Brazil 1 <1%
Australia 1 <1%
Belgium 1 <1%
Other 3 1%
Unknown 245 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 58 22%
Student > Master 54 21%
Researcher 48 18%
Student > Bachelor 20 8%
Student > Doctoral Student 13 5%
Other 31 12%
Unknown 39 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 73 28%
Biochemistry, Genetics and Molecular Biology 58 22%
Engineering 36 14%
Chemical Engineering 20 8%
Chemistry 16 6%
Other 17 6%
Unknown 43 16%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 May 2022.
All research outputs
#7,960,052
of 25,373,627 outputs
Outputs from Biotechnology & Bioengineering
#2,393
of 6,450 outputs
Outputs of similar age
#54,382
of 178,785 outputs
Outputs of similar age from Biotechnology & Bioengineering
#22
of 52 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 6,450 research outputs from this source. They receive a mean Attention Score of 4.8. This one has gotten more attention than average, scoring higher than 62% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 178,785 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 52 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 57% of its contemporaries.