| Title |
Protection induced by malaria virus-like particles containing codon-optimized AMA-1 of Plasmodium berghei
|
|---|---|
| Published in |
Malaria Journal, December 2019
|
| DOI | 10.1186/s12936-019-3017-2 |
| Pubmed ID | |
| Authors |
Dong-Hun Lee, Ki-Back Chu, Hae-Ji Kang, Su-Hwa Lee, Manika Chopra, Hyo-Jick Choi, Eun-Kyung Moon, Kyung-Soo Inn, Fu-Shi Quan |
| Abstract |
Despite the extensive endeavours, developing an effective malaria vaccine remains as a great challenge. Apical membrane antigen 1 (AMA-1) located on the merozoite surface of parasites belonging to the genus Plasmodium is involved in red blood cell invasion. Influenza virus-like particle (VLP) vaccines containing codon-optimized or native (non-codon optimized) AMA-1 from Plasmodium berghei were generated. VLP-induced protective immunity was evaluated in a mouse model. Mice immunized with VLP vaccine containing the codon-optimized AMA-1 elicited higher levels of P. berghei-specific IgG and IgG2a antibody responses compared to VLPs containing non-codon optimized AMA-1 before and after challenge infection. Codon-optimized AMA-1 VLP vaccination induced higher levels of CD4+ T cells, CD8+ T cells, B cells, and germinal centre cell responses compared to non-codon optimized AMA-1 VLPs. Importantly, the codon-optimized AMA-1 VLP vaccination showed lower body weight loss, longer survival and a significant decrease in parasitaemia compared to non-codon optimized VLP vaccination. Overall, VLP vaccine expressing codon-optimized AMA-1 induced better protective efficacy than VLPs expressing the non-codon optimized AMA-1. Current findings highlight the importance of codon-optimization for vaccine use and its potential involvement in future malaria vaccine design strategies. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 19 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 7 | 37% |
| Researcher | 3 | 16% |
| Student > Ph. D. Student | 2 | 11% |
| Professor | 1 | 5% |
| Student > Postgraduate | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 5 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 4 | 21% |
| Biochemistry, Genetics and Molecular Biology | 3 | 16% |
| Computer Science | 1 | 5% |
| Nursing and Health Professions | 1 | 5% |
| Medicine and Dentistry | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 8 | 42% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 December 2019.
All research outputs
#18,703,173
of 23,179,757 outputs
Outputs from Malaria Journal
#5,102
of 5,628 outputs
Outputs of similar age
#337,162
of 459,136 outputs
Outputs of similar age from Malaria Journal
#107
of 118 outputs
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