| Title |
Methods for extraction and ex-vivo experimentation with the most complex human commensal, Demodex spp.
|
|---|---|
| Published in |
Experimental and Applied Acarology, December 2019
|
| DOI | 10.1007/s10493-019-00450-9 |
| Pubmed ID | |
| Authors |
Benjamin M. Clanner-Engelshofen, Lars E. French, Markus Reinholz |
| Abstract |
Demodex spp. mites are the most complex organisms of the human skin microbiome and were discovered more than 175 years ago, yet only little basic research is published about them. As they can be pathophysiologically relevant ectoparasites associated with rosacea, pityriasis folliculorum, and other inflammatory skin diseases, more research should be encouraged. Being a large microorganism or a tiny animal, there are no established basic methods to handle these mites. Here, we describe techniques enabling the extraction of Demodex mites from human skin, their analysis in different ex-vivo settings, the lysis of their exoskeleton, their preservation by freezing, and observation microscopically using specific fluorescent dyes or their inherent autofluorescence. These procedures should facilitate future Demodex research and fuel further the generation of knowledge. Furthermore it is intended to ultimatively enable the mite's cultivation in vitro and reveal its pathophysiological mechanisms. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 25% |
| Unknown | 3 | 75% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 1 | 20% |
| Unknown | 4 | 80% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 1 | 20% |
| Unknown | 4 | 80% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 February 2020.
All research outputs
#14,081,606
of 23,849,058 outputs
Outputs from Experimental and Applied Acarology
#403
of 914 outputs
Outputs of similar age
#228,919
of 463,420 outputs
Outputs of similar age from Experimental and Applied Acarology
#9
of 22 outputs
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