Title |
VEGFR2-targeted fusion antibody improved NK cell-mediated immunosurveillance against K562 cells
|
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Published in |
Immunologic Research, May 2016
|
DOI | 10.1007/s12026-016-8800-3 |
Pubmed ID | |
Authors |
Xueyan Ren, Wei Xie, Youfu Wang, Menghuai Xu, Fang Liu, Mingying Tang, Chenchen Li, Min Wang, Juan Zhang |
Abstract |
MHC class I polypeptide-related sequence A (MICA), which is normally expressed on cancer cells, activates NK cells via NK group 2-member D pathway. However, some cancer cells escape NK-mediated immune surveillance by shedding membrane MICA causing immune suppression. To address this issue, we designed an antibody-MICA fusion targeting tumor-specific antigen (vascular endothelial growth factor receptor 2, VEGFR2) based on our patented antibody (mAb04) against VEGFR2. In vitro results demonstrate that the fusion antibody retains both the antineoplastic and the immunomodulatory activity of mAb04. Further, we revealed that it enhanced NK-mediated immunosurveillance against K562 cells through increasing degranulation and cytokine production of NK cells. The overall data suggest our new fusion protein provides a promising approach for cancer-targeted immunotherapy and has prospects for potential application of chronic myeloid leukemia. |
X Demographics
Geographical breakdown
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Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 1 | 25% |
Student > Bachelor | 1 | 25% |
Researcher | 1 | 25% |
Student > Doctoral Student | 1 | 25% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 2 | 50% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 25% |
Chemical Engineering | 1 | 25% |