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Bioinformatic analysis of fruit-specific expressed sequence tag libraries of Diospyros kaki Thunb.: view at the transcriptome at different developmental stages

Overview of attention for article published in 3 Biotech, April 2011
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Title
Bioinformatic analysis of fruit-specific expressed sequence tag libraries of Diospyros kaki Thunb.: view at the transcriptome at different developmental stages
Published in
3 Biotech, April 2011
DOI 10.1007/s13205-011-0005-9
Pubmed ID
Authors

Gaurav Sablok, Chun Luo, Wan Sin Lee, Farzana Rahman, Tatiana V. Tatarinova, Jennifer Ann Harikrishna, Zhengrong Luo

Abstract

We present here a systematic analysis of the Diospyros kaki expressed sequence tags (ESTs) generated from development stage-specific libraries. A total of 2,529 putative tentative unigenes were identified in the MF library whereas the OYF library displayed 3,775 tentative unigenes. Among the two cDNA libraries, 325 EST-Simple sequence repeats (SSRs) in 296 putative unigenes were detected in the MF library showing an occurrence of 11.7% with a frequency of 1 SSR/3.16 kb whereas the OYF library had an EST-SSRs occurrence of 10.8% with 407 EST-SSRs in the 352 putative unigenes with a frequency of 1 SSR/2.92 kb. We observed a higher frequency of SNPs and indels in the OYF library (20.94 SNPs/indels per 100 bp) in comparison to MF library showed a relatively lower frequency (0.74 SNPs/indels per 100 bp). A combined homology and secondary structure analysis approach identified a potential miRNA precursor, an ortholog of miR159, and potential miR159 targets, in the development-specific ESTs of D. kaki. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13205-011-0005-9) contains supplementary material, which is available to authorized users.

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Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 55%
Student > Ph. D. Student 3 27%
Student > Postgraduate 1 9%
Student > Master 1 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 91%
Biochemistry, Genetics and Molecular Biology 1 9%
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Attention Score in Context

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#18,305,773
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Outputs from 3 Biotech
#618
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#94,925
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Outputs of similar age from 3 Biotech
#3
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