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Cytoskeleton Dynamics

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Cover of 'Cytoskeleton Dynamics'

Table of Contents

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    Book Overview
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    Chapter 1 Purification of Mammalian Tubulins and Tubulin-Associated Proteins Using a P2A-Based Expression System
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    Chapter 2 Expression and Purification of Microtubule-Associated Proteins from HEK293T Cells for In Vitro Reconstitution
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    Chapter 3 In Vitro Reconstitution Assays of Microtubule Amplification and Lattice Repair by the Microtubule-Severing Enzymes Katanin and Spastin
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    Chapter 4 In Vitro Microtubule Dynamics Assays Using Dark-Field Microscopy
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    Chapter 5 In Vitro Reconstitution of Dynamic Co-organization of Microtubules and Actin Filaments in Emulsion Droplets
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    Chapter 6 Studying Tau-Microtubule Interaction Using Single-Molecule TIRF Microscopy
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    Chapter 7 In Vitro FRET- and Fluorescence-Based Assays to Study Protein Conformation and Protein-Protein Interactions in Mitosis
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    Chapter 8 Methods for Studying Membrane-Associated Bacterial Cytoskeleton Proteins In Vivo by TIRF Microscopy
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    Chapter 9 Processing TIRF Microscopy Images to Characterize the Dynamics and Morphology of Bacterial Actin-Like Assemblies
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    Chapter 10 Studying Centriole Duplication and Elongation in Human Cells
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    Chapter 11 Assaying Microtubule Nucleation
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    Chapter 12 Detection of Microtubule Nucleation Hotspots at the Golgi
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    Chapter 13 Ultrastructural Analysis of Microtubule Ends
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    Chapter 14 Optogenetic Control of Microtubule Dynamics
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    Chapter 15 Measurement of Microtubule Half-Life and Poleward Flux in the Mitotic Spindle by Photoactivation of Fluorescent Tubulin
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    Chapter 16 Functional Dissection of Mitosis Using Immortalized Fibroblasts from the Indian Muntjac, a Placental Mammal with Only Three Chromosomes
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    Chapter 17 Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes
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    Chapter 18 Using the Four-Cell C. elegans Embryo to Study Contractile Ring Dynamics During Cytokinesis
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    Chapter 19 Knocking Out Multiple Genes in Cultured Primary Neurons to Study Tubulin Posttranslational Modifications
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    Chapter 20 Measuring the Impact of Tubulin Posttranslational Modifications on Axonal Transport
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    Chapter 21 Methods to Measure Perinuclear Actin Dynamics During Nuclear Movement in Migrating Cells
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Title
Cytoskeleton Dynamics
Published by
Springer US, January 2020
DOI 10.1007/978-1-0716-0219-5
ISBNs
978-1-07-160218-8, 978-1-07-160219-5
Editors

Helder Maiato

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