Title |
A Sensitive, Colorimetric, High-Throughput Loop-Mediated Isothermal Amplification Assay for the Detection of Plasmodium knowlesi.
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Published in |
The American Journal of Tropical Medicine and Hygiene, May 2016
|
DOI | 10.4269/ajtmh.15-0670 |
Pubmed ID | |
Authors |
Sumudu Britton, Qin Cheng, Matthew J Grigg, Timothy William, Nicholas M Anstey, James S McCarthy |
Abstract |
The simian parasite Plasmodium knowlesi is now the commonest cause of malaria in Malaysia and can rapidly cause severe and fatal malaria. However, microscopic misdiagnosis of Plasmodium species is common, rapid antigen detection tests remain insufficiently sensitive and confirmation of P. knowlesi requires polymerase chain reaction (PCR). Thus available point-of-care diagnostic tests are inadequate. This study reports the development of a simple, sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay (HtLAMP) diagnostic test using novel primers for the detection of P. knowlesi This assay is able to detect 0.2 parasites/μL, and compared with PCR has a sensitivity of 96% for the detection of P. knowlesi, making it a potentially field-applicable point-of-care diagnostic tool. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 38 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 8 | 21% |
Student > Ph. D. Student | 5 | 13% |
Student > Master | 4 | 11% |
Student > Doctoral Student | 3 | 8% |
Researcher | 3 | 8% |
Other | 6 | 16% |
Unknown | 9 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 8 | 21% |
Biochemistry, Genetics and Molecular Biology | 7 | 18% |
Agricultural and Biological Sciences | 3 | 8% |
Immunology and Microbiology | 2 | 5% |
Mathematics | 1 | 3% |
Other | 6 | 16% |
Unknown | 11 | 29% |