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A Sensitive, Colorimetric, High-Throughput Loop-Mediated Isothermal Amplification Assay for the Detection of Plasmodium knowlesi.

Overview of attention for article published in The American Journal of Tropical Medicine and Hygiene, May 2016
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38 Mendeley
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Title
A Sensitive, Colorimetric, High-Throughput Loop-Mediated Isothermal Amplification Assay for the Detection of Plasmodium knowlesi.
Published in
The American Journal of Tropical Medicine and Hygiene, May 2016
DOI 10.4269/ajtmh.15-0670
Pubmed ID
Authors

Sumudu Britton, Qin Cheng, Matthew J Grigg, Timothy William, Nicholas M Anstey, James S McCarthy

Abstract

The simian parasite Plasmodium knowlesi is now the commonest cause of malaria in Malaysia and can rapidly cause severe and fatal malaria. However, microscopic misdiagnosis of Plasmodium species is common, rapid antigen detection tests remain insufficiently sensitive and confirmation of P. knowlesi requires polymerase chain reaction (PCR). Thus available point-of-care diagnostic tests are inadequate. This study reports the development of a simple, sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay (HtLAMP) diagnostic test using novel primers for the detection of P. knowlesi This assay is able to detect 0.2 parasites/μL, and compared with PCR has a sensitivity of 96% for the detection of P. knowlesi, making it a potentially field-applicable point-of-care diagnostic tool.

X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 38 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 8 21%
Student > Ph. D. Student 5 13%
Student > Master 4 11%
Student > Doctoral Student 3 8%
Researcher 3 8%
Other 6 16%
Unknown 9 24%
Readers by discipline Count As %
Medicine and Dentistry 8 21%
Biochemistry, Genetics and Molecular Biology 7 18%
Agricultural and Biological Sciences 3 8%
Immunology and Microbiology 2 5%
Mathematics 1 3%
Other 6 16%
Unknown 11 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 May 2016.
All research outputs
#17,289,387
of 25,377,790 outputs
Outputs from The American Journal of Tropical Medicine and Hygiene
#7,613
of 9,524 outputs
Outputs of similar age
#194,175
of 315,795 outputs
Outputs of similar age from The American Journal of Tropical Medicine and Hygiene
#43
of 88 outputs
Altmetric has tracked 25,377,790 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 9,524 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.6. This one is in the 15th percentile – i.e., 15% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 315,795 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 88 others from the same source and published within six weeks on either side of this one. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.