| Title |
In Vitro Culture of Mouse Embryos Reduces Differential Gene Expression Between Inner Cell Mass and Trophectoderm
|
|---|---|
| Published in |
Reproductive Sciences, December 2012
|
| DOI | 10.1177/1933719111428522 |
| Pubmed ID | |
| Authors |
G. Giritharan, L. Delle Piane, A. Donjacour, F. J. Esteban, J. A. Horcajadas, E. Maltepe, P. Rinaudo |
| Abstract |
Differences in gene expression and imprinting have been reported, comparing in vivo versus in vitro generated preimplantation embryos. Furthermore, mouse studies have shown that placenta development is altered following in vitro culture. However, the molecular mechanisms underlying these findings are unknown. We therefore isolated trophectoderm (TE) and inner cell mass (ICM) cells from in vivo and in vitro fertilization (IVF) embryos and evaluated their transcriptome using microarrays. We found that the transcriptomes of in vitro produced ICM and TE cells showed remarkably few differences compared to ICM and TE cells of in vivo generated embryos. In vitro fertilization embryos showed a reduced number of TE cells compared to in vivo embryos. In addition, TE of IVF embryos showed significant downregulation of solute transporter genes and of genes involved in placenta formation (Eomesodermin, Socs3) or implantation (Hbegf). In summary, IVF and embryo culture significantly affects the transcriptome of ICM and TE cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 5% |
| Mexico | 1 | 3% |
| Australia | 1 | 3% |
| Unknown | 33 | 89% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 19% |
| Researcher | 7 | 19% |
| Student > Master | 6 | 16% |
| Student > Postgraduate | 3 | 8% |
| Student > Bachelor | 2 | 5% |
| Other | 8 | 22% |
| Unknown | 4 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 27% |
| Agricultural and Biological Sciences | 10 | 27% |
| Social Sciences | 4 | 11% |
| Medicine and Dentistry | 3 | 8% |
| Chemical Engineering | 1 | 3% |
| Other | 3 | 8% |
| Unknown | 6 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 April 2012.
All research outputs
#15,243,120
of 22,664,644 outputs
Outputs from Reproductive Sciences
#498
of 1,202 outputs
Outputs of similar age
#181,212
of 280,340 outputs
Outputs of similar age from Reproductive Sciences
#22
of 48 outputs
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So far Altmetric has tracked 1,202 research outputs from this source. They receive a mean Attention Score of 5.0. This one is in the 48th percentile – i.e., 48% of its peers scored the same or lower than it.
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We're also able to compare this research output to 48 others from the same source and published within six weeks on either side of this one. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.